Fig. 3.

EMSA of eGFP-gp16 on terminally blocked short dsDNA. 40 bp Cy3-dsDNA, with biotin attached to each end, was incubated with eGFP-gp16, non-hydrolyzable γ-S-ATP, and streptavidin in different combinations. The complexes that were mixed at approximately a 6:1 molar ratio of protein:DNA were then electrophoresed through an agarose gel and scanned for Cy3 fluorescence of DNA and GFP fluorescence of gp16 (see Materials and Methods).