Figure 2.

Sequence Alignment of RLIP76 with RhoGAP Domains Whose Structures Have Been Solved

The positions of the RLIP76 α helices are denoted as gray cylinders above the alignment and are labeled according to the names of the equivalent helices in the p50 RhoGAP protein (Barrett et al., 1997). The helical regions in all the proteins are shaded gray and boxed. Residues involved in contacts with Cdc42 are marked by circles above the p50 RhoGAP sequence colored as follows: white circles: in contact in the Cdc42·GMPPNP complex (PDB ID code 1AM4); black circles: in contact in the Cdc42·GDP·AlF₃ complex (PDB ID code 1GRN); gray circles: in contact in both complexes. The Arg finger and secondary Lys are marked by black and white triangles, respectively. Residues in the loop between helices αF and αG are shown in white font on a black background if their backbone B factors in the X-ray structures are more than 20 Å² higher than the average or whose coordinates are missing. For the NMR structure of RhoGAP5, residues with a backbone rmsd of more than 2 Å are highlighted in the same manner. The two vertical lines between helices A0 and A mark the position of a large insertion in OCRL, which has been omitted for clarity. The alignment was generated using T-Coffee (Di Tommaso et al., 2011) and Top3d (Winn et al., 2011) and the figure was produced using ALSCRIPT (Barton, 1993). The PDB ID codes for the structures are shown after the name of each protein.