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. 1996 Aug 20;93(17):8804–8808. doi: 10.1073/pnas.93.17.8804

Targeted transgenesis.

M Jasin 1, M E Moynahan 1, C Richardson 1
PMCID: PMC38547  PMID: 8799106

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Selected References

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  1. Askew G. R., Doetschman T., Lingrel J. B. Site-directed point mutations in embryonic stem cells: a gene-targeting tag-and-exchange strategy. Mol Cell Biol. 1993 Jul;13(7):4115–4124. doi: 10.1128/mcb.13.7.4115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bartolomei M. S., Webber A. L., Brunkow M. E., Tilghman S. M. Epigenetic mechanisms underlying the imprinting of the mouse H19 gene. Genes Dev. 1993 Sep;7(9):1663–1673. doi: 10.1101/gad.7.9.1663. [DOI] [PubMed] [Google Scholar]
  3. Blom van Assendelft G., Hanscombe O., Grosveld F., Greaves D. R. The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner. Cell. 1989 Mar 24;56(6):969–977. doi: 10.1016/0092-8674(89)90630-2. [DOI] [PubMed] [Google Scholar]
  4. Bradley A., Evans M., Kaufman M. H., Robertson E. Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines. Nature. 1984 May 17;309(5965):255–256. doi: 10.1038/309255a0. [DOI] [PubMed] [Google Scholar]
  5. Brandon E. P., Idzerda R. L., McKnight G. S. Knockouts. Targeting the mouse genome: a compendium of knockouts (Part I) Curr Biol. 1995 Jun 1;5(6):625–634. doi: 10.1016/s0960-9822(95)00127-8. [DOI] [PubMed] [Google Scholar]
  6. Brandon E. P., Idzerda R. L., McKnight G. S. Targeting the mouse genome: a compendium of knockouts (Part II) Curr Biol. 1995 Jul 1;5(7):758–765. doi: 10.1016/s0960-9822(95)00152-7. [DOI] [PubMed] [Google Scholar]
  7. Brandon E. P., Idzerda R. L., McKnight G. S. Targeting the mouse genome: a compendium of knockouts (Part III) Curr Biol. 1995 Aug 1;5(8):873–881. doi: 10.1016/s0960-9822(95)00177-1. [DOI] [PubMed] [Google Scholar]
  8. Braun R. E., Lo D., Pinkert C. A., Widera G., Flavell R. A., Palmiter R. D., Brinster R. L. Infertility in male transgenic mice: disruption of sperm development by HSV-tk expression in postmeiotic germ cells. Biol Reprod. 1990 Oct;43(4):684–693. doi: 10.1095/biolreprod43.4.684. [DOI] [PubMed] [Google Scholar]
  9. Brinster R. L., Chen H. Y., Trumbauer M., Senear A. W., Warren R., Palmiter R. D. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell. 1981 Nov;27(1 Pt 2):223–231. doi: 10.1016/0092-8674(81)90376-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Bronson S. K., Plaehn E. G., Kluckman K. D., Hagaman J. R., Maeda N., Smithies O. Single-copy transgenic mice with chosen-site integration. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9067–9072. doi: 10.1073/pnas.93.17.9067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Collignon J., Varlet I., Robertson E. J. Relationship between asymmetric nodal expression and the direction of embryonic turning. Nature. 1996 May 9;381(6578):155–158. doi: 10.1038/381155a0. [DOI] [PubMed] [Google Scholar]
  12. Corral J., Lavenir I., Impey H., Warren A. J., Forster A., Larson T. A., Bell S., McKenzie A. N., King G., Rabbitts T. H. An Mll-AF9 fusion gene made by homologous recombination causes acute leukemia in chimeric mice: a method to create fusion oncogenes. Cell. 1996 Jun 14;85(6):853–861. doi: 10.1016/s0092-8674(00)81269-6. [DOI] [PubMed] [Google Scholar]
  13. Costantini F., Lacy E. Introduction of a rabbit beta-globin gene into the mouse germ line. Nature. 1981 Nov 5;294(5836):92–94. doi: 10.1038/294092a0. [DOI] [PubMed] [Google Scholar]
  14. Doetschman T., Gregg R. G., Maeda N., Hooper M. L., Melton D. W., Thompson S., Smithies O. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature. 1987 Dec 10;330(6148):576–578. doi: 10.1038/330576a0. [DOI] [PubMed] [Google Scholar]
  15. Evans M. J., Kaufman M. H. Establishment in culture of pluripotential cells from mouse embryos. Nature. 1981 Jul 9;292(5819):154–156. doi: 10.1038/292154a0. [DOI] [PubMed] [Google Scholar]
  16. Fukushige S., Sauer B. Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):7905–7909. doi: 10.1073/pnas.89.17.7905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gordon J. W., Ruddle F. H. Integration and stable germ line transmission of genes injected into mouse pronuclei. Science. 1981 Dec 11;214(4526):1244–1246. doi: 10.1126/science.6272397. [DOI] [PubMed] [Google Scholar]
  18. Gordon J. W., Scangos G. A., Plotkin D. J., Barbosa J. A., Ruddle F. H. Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7380–7384. doi: 10.1073/pnas.77.12.7380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Gossler A., Doetschman T., Korn R., Serfling E., Kemler R. Transgenesis by means of blastocyst-derived embryonic stem cell lines. Proc Natl Acad Sci U S A. 1986 Dec;83(23):9065–9069. doi: 10.1073/pnas.83.23.9065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Grosveld F., van Assendelft G. B., Greaves D. R., Kollias G. Position-independent, high-level expression of the human beta-globin gene in transgenic mice. Cell. 1987 Dec 24;51(6):975–985. doi: 10.1016/0092-8674(87)90584-8. [DOI] [PubMed] [Google Scholar]
  21. Gu H., Marth J. D., Orban P. C., Mossmann H., Rajewsky K. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science. 1994 Jul 1;265(5168):103–106. doi: 10.1126/science.8016642. [DOI] [PubMed] [Google Scholar]
  22. Hanks M., Wurst W., Anson-Cartwright L., Auerbach A. B., Joyner A. L. Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2. Science. 1995 Aug 4;269(5224):679–682. doi: 10.1126/science.7624797. [DOI] [PubMed] [Google Scholar]
  23. Hooper M., Hardy K., Handyside A., Hunter S., Monk M. HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells. Nature. 1987 Mar 19;326(6110):292–295. doi: 10.1038/326292a0. [DOI] [PubMed] [Google Scholar]
  24. Jaenisch R. Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1260–1264. doi: 10.1073/pnas.73.4.1260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Jaenisch R. Transgenic animals. Science. 1988 Jun 10;240(4858):1468–1474. doi: 10.1126/science.3287623. [DOI] [PubMed] [Google Scholar]
  26. Jasin M., Berg P. Homologous integration in mammalian cells without target gene selection. Genes Dev. 1988 Nov;2(11):1353–1363. doi: 10.1101/gad.2.11.1353. [DOI] [PubMed] [Google Scholar]
  27. Jasin M. Genetic manipulation of genomes with rare-cutting endonucleases. Trends Genet. 1996 Jun;12(6):224–228. doi: 10.1016/0168-9525(96)10019-6. [DOI] [PubMed] [Google Scholar]
  28. Kadesch T., Berg P. Effects of the position of the simian virus 40 enhancer on expression of multiple transcription units in a single plasmid. Mol Cell Biol. 1986 Jul;6(7):2593–2601. doi: 10.1128/mcb.6.7.2593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Kim C. G., Epner E. M., Forrester W. C., Groudine M. Inactivation of the human beta-globin gene by targeted insertion into the beta-globin locus control region. Genes Dev. 1992 Jun;6(6):928–938. doi: 10.1101/gad.6.6.928. [DOI] [PubMed] [Google Scholar]
  30. Kuehn M. R., Bradley A., Robertson E. J., Evans M. J. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature. 1987 Mar 19;326(6110):295–298. doi: 10.1038/326295a0. [DOI] [PubMed] [Google Scholar]
  31. Le Mouellic H., Lallemand Y., Brûlet P. Targeted replacement of the homeobox gene Hox-3.1 by the Escherichia coli lacZ in mouse chimeric embryos. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4712–4716. doi: 10.1073/pnas.87.12.4712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Li M., Sendtner M., Smith A. Essential function of LIF receptor in motor neurons. Nature. 1995 Dec 14;378(6558):724–727. doi: 10.1038/378724a0. [DOI] [PubMed] [Google Scholar]
  33. Liang F., Romanienko P. J., Weaver D. T., Jeggo P. A., Jasin M. Chromosomal double-strand break repair in Ku80-deficient cells. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8929–8933. doi: 10.1073/pnas.93.17.8929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Lin F. L., Sperle K., Sternberg N. Recombination in mouse L cells between DNA introduced into cells and homologous chromosomal sequences. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1391–1395. doi: 10.1073/pnas.82.5.1391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Magin T. M., McWhir J., Melton D. W. A new mouse embryonic stem cell line with good germ line contribution and gene targeting frequency. Nucleic Acids Res. 1992 Jul 25;20(14):3795–3796. doi: 10.1093/nar/20.14.3795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Mansour S. L., Thomas K. R., Capecchi M. R. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature. 1988 Nov 24;336(6197):348–352. doi: 10.1038/336348a0. [DOI] [PubMed] [Google Scholar]
  37. Mansour S. L., Thomas K. R., Deng C. X., Capecchi M. R. Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7688–7692. doi: 10.1073/pnas.87.19.7688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Martin G. R. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634–7638. doi: 10.1073/pnas.78.12.7634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Mountford P. S., Smith A. G. Internal ribosome entry sites and dicistronic RNAs in mammalian transgenesis. Trends Genet. 1995 May;11(5):179–184. doi: 10.1016/S0168-9525(00)89040-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Mountford P., Zevnik B., Düwel A., Nichols J., Li M., Dani C., Robertson M., Chambers I., Smith A. Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression. Proc Natl Acad Sci U S A. 1994 May 10;91(10):4303–4307. doi: 10.1073/pnas.91.10.4303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. No D., Yao T. P., Evans R. M. Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3346–3351. doi: 10.1073/pnas.93.8.3346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Palmiter R. D., Brinster R. L., Hammer R. E., Trumbauer M. E., Rosenfeld M. G., Birnberg N. C., Evans R. M. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature. 1982 Dec 16;300(5893):611–615. doi: 10.1038/300611a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Palmiter R. D., Sandgren E. P., Avarbock M. R., Allen D. D., Brinster R. L. Heterologous introns can enhance expression of transgenes in mice. Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):478–482. doi: 10.1073/pnas.88.2.478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Ramírez-Solis R., Liu P., Bradley A. Chromosome engineering in mice. Nature. 1995 Dec 14;378(6558):720–724. doi: 10.1038/378720a0. [DOI] [PubMed] [Google Scholar]
  45. Robertson E., Bradley A., Kuehn M., Evans M. Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector. Nature. 1986 Oct 2;323(6087):445–448. doi: 10.1038/323445a0. [DOI] [PubMed] [Google Scholar]
  46. Rouet P., Smih F., Jasin M. Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease. Mol Cell Biol. 1994 Dec;14(12):8096–8106. doi: 10.1128/mcb.14.12.8096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Schwartzberg P. L., Goff S. P., Robertson E. J. Germ-line transmission of a c-abl mutation produced by targeted gene disruption in ES cells. Science. 1989 Nov 10;246(4931):799–803. doi: 10.1126/science.2554496. [DOI] [PubMed] [Google Scholar]
  48. Schwenk F., Baron U., Rajewsky K. A cre-transgenic mouse strain for the ubiquitous deletion of loxP-flanked gene segments including deletion in germ cells. Nucleic Acids Res. 1995 Dec 25;23(24):5080–5081. doi: 10.1093/nar/23.24.5080. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Shaw-White J. R., Denko N., Albers L., Doetschman T. C., Stringer J. R. Expression of the lacZ gene targeted to the HPRT locus in embryonic stem cells and their derivatives. Transgenic Res. 1993 Jan;2(1):1–13. doi: 10.1007/BF01977675. [DOI] [PubMed] [Google Scholar]
  50. Shockett P. E., Schatz D. G. Diverse strategies for tetracycline-regulated inducible gene expression. Proc Natl Acad Sci U S A. 1996 May 28;93(11):5173–5176. doi: 10.1073/pnas.93.11.5173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Smith A. J., De Sousa M. A., Kwabi-Addo B., Heppell-Parton A., Impey H., Rabbitts P. A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP recombination. Nat Genet. 1995 Apr;9(4):376–385. doi: 10.1038/ng0495-376. [DOI] [PubMed] [Google Scholar]
  52. Smithies O., Gregg R. G., Boggs S. S., Koralewski M. A., Kucherlapati R. S. Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination. Nature. 1985 Sep 19;317(6034):230–234. doi: 10.1038/317230a0. [DOI] [PubMed] [Google Scholar]
  53. Stacey A., Schnieke A., McWhir J., Cooper J., Colman A., Melton D. W. Use of double-replacement gene targeting to replace the murine alpha-lactalbumin gene with its human counterpart in embryonic stem cells and mice. Mol Cell Biol. 1994 Feb;14(2):1009–1016. doi: 10.1128/mcb.14.2.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  54. Tajbakhsh S., Buckingham M. E. Mouse limb muscle is determined in the absence of the earliest myogenic factor myf-5. Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):747–751. doi: 10.1073/pnas.91.2.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. Talbot D., Descombes P., Schibler U. The 5' flanking region of the rat LAP (C/EBP beta) gene can direct high-level, position-independent, copy number-dependent expression in multiple tissues in transgenic mice. Nucleic Acids Res. 1994 Mar 11;22(5):756–766. doi: 10.1093/nar/22.5.756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  56. Thomas K. R., Capecchi M. R. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell. 1987 Nov 6;51(3):503–512. doi: 10.1016/0092-8674(87)90646-5. [DOI] [PubMed] [Google Scholar]
  57. Van Deursen J., Fornerod M., Van Rees B., Grosveld G. Cre-mediated site-specific translocation between nonhomologous mouse chromosomes. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7376–7380. doi: 10.1073/pnas.92.16.7376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  58. Vassalli A., Matzuk M. M., Gardner H. A., Lee K. F., Jaenisch R. Activin/inhibin beta B subunit gene disruption leads to defects in eyelid development and female reproduction. Genes Dev. 1994 Feb 15;8(4):414–427. doi: 10.1101/gad.8.4.414. [DOI] [PubMed] [Google Scholar]
  59. Wagner E. F., Stewart T. A., Mintz B. The human beta-globin gene and a functional viral thymidine kinase gene in developing mice. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5016–5020. doi: 10.1073/pnas.78.8.5016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  60. Wagner T. E., Hoppe P. C., Jollick J. D., Scholl D. R., Hodinka R. L., Gault J. B. Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6376–6380. doi: 10.1073/pnas.78.10.6376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  61. Weiler-Guettler H., Aird W. C., Husain M., Rayburn H., Rosenberg R. D. Targeting of transgene expression to the vascular endothelium of mice by homologous recombination at the thrombomodulin locus. Circ Res. 1996 Feb;78(2):180–187. doi: 10.1161/01.res.78.2.180. [DOI] [PubMed] [Google Scholar]
  62. Wu C. L., Melton D. W. Production of a model for Lesch-Nyhan syndrome in hypoxanthine phosphoribosyltransferase-deficient mice. Nat Genet. 1993 Mar;3(3):235–240. doi: 10.1038/ng0393-235. [DOI] [PubMed] [Google Scholar]
  63. Wu H., Liu X., Jaenisch R. Double replacement: strategy for efficient introduction of subtle mutations into the murine Col1a-1 gene by homologous recombination in embryonic stem cells. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2819–2823. doi: 10.1073/pnas.91.7.2819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  64. Wurst W., Rossant J., Prideaux V., Kownacka M., Joyner A., Hill D. P., Guillemot F., Gasca S., Cado D., Auerbach A. A large-scale gene-trap screen for insertional mutations in developmentally regulated genes in mice. Genetics. 1995 Feb;139(2):889–899. doi: 10.1093/genetics/139.2.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  65. Xuan S., Baptista C. A., Balas G., Tao W., Soares V. C., Lai E. Winged helix transcription factor BF-1 is essential for the development of the cerebral hemispheres. Neuron. 1995 Jun;14(6):1141–1152. doi: 10.1016/0896-6273(95)90262-7. [DOI] [PubMed] [Google Scholar]
  66. Zhou Q. Y., Palmiter R. D. Dopamine-deficient mice are severely hypoactive, adipsic, and aphagic. Cell. 1995 Dec 29;83(7):1197–1209. doi: 10.1016/0092-8674(95)90145-0. [DOI] [PubMed] [Google Scholar]
  67. Zijlstra M., Li E., Sajjadi F., Subramani S., Jaenisch R. Germ-line transmission of a disrupted beta 2-microglobulin gene produced by homologous recombination in embryonic stem cells. Nature. 1989 Nov 23;342(6248):435–438. doi: 10.1038/342435a0. [DOI] [PubMed] [Google Scholar]
  68. Zou Y. R., Gu H., Rajewsky K. Generation of a mouse strain that produces immunoglobulin kappa chains with human constant regions. Science. 1993 Nov 19;262(5137):1271–1274. doi: 10.1126/science.8235658. [DOI] [PubMed] [Google Scholar]
  69. te Riele H., Maandag E. R., Berns A. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5128–5132. doi: 10.1073/pnas.89.11.5128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  70. te Riele H., Maandag E. R., Clarke A., Hooper M., Berns A. Consecutive inactivation of both alleles of the pim-1 proto-oncogene by homologous recombination in embryonic stem cells. Nature. 1990 Dec 13;348(6302):649–651. doi: 10.1038/348649a0. [DOI] [PubMed] [Google Scholar]

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