. Author manuscript; available in PMC: 2014 Jul 23.

Published in final edited form as: Biochemistry. 2013 Jul 15;52(29):10.1021/bi400320s. doi: 10.1021/bi400320s

Table 5.

Fitting parameters for the change in Van't Hoff enthalpy determined by VTCD.

	[NaCl] (mM)	ΔH_v1 (kcal mol^-1)	ΔH_v2 (kcal mol^-1)
N-NFU	100.0	69.6 ± 6.1
N-NFU	0.0	41.8 ± 4.5
C-NFU	100.0	13.6 ± 3.2
C-NFU	0.0	17.7 ± 0.9
N-NFU / C-NFU	100.0	43.3 ± 4.1	69.2 ± 1.1
N-NFU / C-NFU	0.0	44.1 ± 4.5	45.8 ± 0.7
NFU	100.0	23.9 ± 1.7	41.0 ± 2.7
NFU	0.0	31.8 ± 6.3	28.3 ± 3.3

Values were obtained from fitting the molar ellipticity versus temperature by use of equations 1 or 2. Increasing ionic strength resulted in an increase in the Van't Hoff enthalpy, indicating a relatively more stable secondary structure for well-folded domains, such as N-NFU, and consistent with the trends observed in the melting temperatures defined in Table 4.