Table 3.

Dependence on 5′ Mismatches: Strand Incision Rates of APE1 Acting on AP, AP-Red, or THF DNA^a,^b

	kchemistry (s−1)a,b
Substrate	MM-1	MM-2	MM-3	MM-4
AP	156 ± 29	312 ± 29	≥700	≥700
AP-Red	163 ± 21	307 ± 24	≥700	≥700
THF	116 ± 29c	194 ± 32d	575 ± 65	≥700

^aMeasured at 37 °C under transient-state conditions using RQF instrumentation.

^bError represents the standard deviation from at least four experiments.

^cp=0.04 when compared to AP DNA using student’s T test.

^dp=0.008 when compared to AP DNA using student’s T test.