Table II. List of proteins involved in mitochondrial protein transport, biogenesis and regulation of crista morphology. The data shown are the average ratios (HFD vs. ND) from three biological replicates. Also shown are the number of unique peptides identified for each protein (unique_Pep) and number of PQD spectrum (Spectrum_num) used for protein quantitation. The protein coverage is shown as Protein_Coverage. ND: not detected.

Gene Symbol	Accession	set1_HFD/ND	set2_HFD/ND	set3_HFD/ND	Average HFD/ND	p value	Unique_Peptide	Spectrum_num	Protein_Coverage	Protein
Chchd3	IPI00754739	2.10	1.10	3.68	2.29	0.18	6	18	0.38	Coiled-coil-helix coiled-coil-helix domain- containing protein 3
Samm50	IPI00221608	0.96	3.08	3.90	2.64	0.20	6	24	0.10	Sorting and assembly machinery component 50 homolog
Immt	IPI00554845	1.39	4.75	2.96	3.03	0.11	26	149	0.38	Mitofilin Isoform 5 of Mitochondrial inner membrane protein
Mtx1	IPI00112327	4.26	ND	8.21	6.24	0.20	4	7	0.14	Metaxin-1
Opa1	IPI00403336	2.15	1.76	2.61	2.17	0.02	12	22	0.13	Optic atrophy 1
Timm44	IPI00135068	1.22	2.67	7.03	3.64	0.18	9	21	0.18	Import inner membrane translocase subunit TIM44
Timm17b	IPI00129504	2.59	ND	2.48	2.54	0.18	2	5	0.17	Mitochondrial import inner membrane translocase subunit Tim17-B
Hspd1	IPI00308885	1.19	1.54	2.98	1.90	0.18	43	1003	0.55	Hsp60 Isoform 1 of 60 kDa heat shock protein
Hspa9	IPI00133903	1.62	1.60	3.96	2.39	0.12	34	947	0.49	Hspa9 Stress-70 protein
Timm8a1	IPI00125776	0.88	1.62	4.57	2.36	0.32	2	33	0.23	Mitochondrial import inner membrane translocase subunit Tim8 A
Timm13	IPI00134484	2.38	2.06	2.55	2.33	0.01	4	123	0.37	Mitochondrial import inner membrane translocase subunit Tim13