Analysis of the Secretome and Identification of Novel Constituents From Culture Filtrate of Bacillus Calmette-Guerin Using High-resolution Mass Spectrometry

Vol. 12 (2013) 2081–2095

The original article failed to cite the article Bell, et al, 2012 J Proteome Res 11, 119–130.

Page 2081, abstract: In the sentence on line 23, “There are 103 secreted proteins that have not been reported in previous studies on the mycobacterial secretome and are unique to our study,” the word “the mycobacterial” should be revised to “BCG”.

Page 2082, left column: In the end of first paragraph, the following sentence should be added. “Bell et al. used a variety of methods to study M. tuberculosis proteins by LC-MS and created a comprehensive resource by characterizing all M. tuberculosis subcellular fractions. In total 398 proteins were included in the CF fraction with high probability (74)”.

Page 2082, right column: In the sentence on line 10, “We found 103 secreted proteins that have not been reported in previous studies on the mycobacterial secretome and that are unique to our study”, the word “the mycobacterial” should be revised to “BCG”.

Page 2086, left column, the third paragraph: Since we integrated the identification data in CF fraction of M. tuberculosis from Dr. Hess work, some data in the “Comparisons with Other Studies on the Mycobacterial Secretome” paragraph should be revised accordingly. The first six sentences, “To investigate the secreted proteins in CFs, a number of mycobacterial secretome studies have been undertaken (8, 11–14, 25–34). Table I summarizes the major studies on the mycobacterial secretome performed to date. These studies focused on CFs of different mycobacterial substrains, including BCG variants. Combining all of the data from the studies published to date, there are 397 proteins that have been reported in different mycobacterial culture filtrates. Among them, 148 proteins were considered to be secreted proteins, including classical and leaderless secreted proteins or lipoproteins. We compared the proteins identified in our study with those identified in previous studies and found that 82 of the secreted proteins were previously reported. Therefore, there are 103 secreted proteins that have not been reported previously and are unique to our study”, should be revised like this: “To investigate the secreted proteins in CFs, a number of mycobacterial secretome studies have been undertaken (8, 11–14, 25–34, 74). Table I summarizes the major studies on the mycobacterial secretome performed to date. These studies focused on CFs of different mycobacterial substrains, including BCG variants. Combining all of the data from the studies published to date, there are 592 proteins that have been reported in different mycobacterial CFs. Among them, 179 proteins were considered to be secreted proteins, including classical and leaderless secreted proteins or lipoproteins. We compared the proteins identified in our study with those identified in previous studies in mycobacterial secretome and found that 113 of the secreted proteins were previously reported. Therefore, there are 72 secreted proteins that have not been reported previously in mycobacterial secretome. Comparing with BCG secretome, 103 secreted proteins have not been reported previously in CFs of BCG and are unique to our study”.

Table I. Summary of the major mass spectrometry-based studies performed on mycobacterial secretome to date.

Table includes details about the number of total proteins and secreted proteins, the analysis method, and the type of mass spectrometer used in the listed studies.

Year	Title	No. of identifications	No. of secreted proteins	Analysis method and instrument used	Reference
1997	Definition of Mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and electrospray mass spectrometry	32	n.d.	2-DE and TSQ-700 quadrupole MS	(25)
1999	Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains: towards functional genomics of microbial pathogens	263	54	2-DE and MALDI-TOF (Voyager Elite MS)	(26)
2000	Toward the proteome of Mycobacterium tuberculosis	288	49	2-DE and MALDI-MS	(27)
2000	Mapping and identification of Mycobacterium tuberculosis proteins by two-dimensional gel electrophoresis, microsequencing and immunodetection	61	14	2-DE and microsequencing, immunodetection	(28)
2001	The application of proteomics in defining the T cell antigens of Mycobacterium tuberculosis	30	n.d.	2-DE and LCQ-ESI-MS	(29)
2002	Hypoxic response of Mycobacterium tuberculosis studied by metabolic labeling and proteome analysis of cellular and extracellular proteins	7	2	2-DE and LCQ-MS	(30)
2003	Comparative proteome analysis of culture supernatant proteins from virulent Mycobacterium tuberculosis H37Rv and attenuated M. bovis BCG Copenhagen	137	42	MALDI-MS PMF, ESI-MS/MS or CapLC-ESI-MS/MS	(8)
2003	Comparative proteome analysis of culture supernatant proteins of Mycobacterium tuberculosis H37Rv and H37Ra	5	n.d.	2-DE-MALDI-TOF MS	(31)
2003	Identification of novel proteins in culture filtrates of Mycobacterium bovis bacillus Calmette-Guérin in the isoelectric point range 6–11	12	n.d.	2-DE and MALDI-TOF (Voyager Spec MS)	(12)
2004	CFP10 discriminates between nonacetylated and acetylated ESAT-6 of Mycobacterium tuberculosis by differential interaction	8	4	1-DE and MALDI-MS and ESI-MS/MS (Voyager Elite)	(10)
2007	Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv	257	159	2-DE and MALDI-TOF MS; LC coupled MS/MS.	(11)
2009	Immunoproteomic identification of secretory and subcellular protein antigens and functional evaluation of the secretome fraction of Mycobacterium immunogenum, a newly recognized species of the Mycobacterium chelonae-Mycobacterium abscessus group	33	4	2-DE and MALDI-TOF	(33)
2009	Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins	41	31	2-DE, Ligand Blotting and Immunoblotting, LC-3200 Q TRAP-MS	(32)
2010	The secretome of a recombinant BCG substrain reveals differences in hypothetical proteins	9	n.d.	2DE-3200 Q-TRAP-MS/MS	(13)
2010	Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis	101	n.d.	2-DE,iTRAQ-labeled and LTQ-MS	(34)
2011	Proteomic profile of culture filtrate from the Brazilian vaccine strain Mycobacterium bovis BCG Moreau compared to M. bovis BCG Pasteur	101	53	2-DE and MALDI-TOF/TOF (4700 Proteomics Analyzer)	(14)
2012	Characterization of the Mycobacterium tuberculosis proteome by liquid chromatography mass spectrometry-based proteomics techniques: a comprehensive resource for tuberculosis research	398	n.d.	LC and ESI-MSMS (LTQ-Orbitrap Classic)	(74)
2013	Analysis of the secretome and identification of novel constituents from culture filtrate of bacillus Calmette-Guérin using high-resolution mass spectrometry	239	185	1-DE and ESI-MSMS (LTQ-Orbitrap Velos)	This study

ESI, electrospray ionization; PMF, peptide mass fingerprint; n.d., no data.

Page 2093, left column: In the sentence on line 15 in CONCLUSIONS section, “These data represent the largest number of mycobacterial secreted proteins reported in a single study, and some of these proteins might be potential candidates for vaccination and therapeutics”, the word “mycobacterial” should be revised to “BCG”.