Abstract
The response of peritoneal exudate lymphocytes to allogeneic tumor cells was used to determine whether the in vivo generation of cytotoxic T cells (CTL) involved the proliferation of precursor cells. Ten days post-injection, both cytotoxic activity and the formation of conjugates between lymphocytes and target cells were shown to be specific for the immunizing tumor alloantigens and to be effected by Ly-2+ cells. A cell-sorting-based procedure was developed to isolate specific conjugates between red-fluorescence-tagged CTL and blue-fluorescence-tagged tumor target cells. When [3H]thymidine was administered during the response, almost all isolated conjugate-forming CTL were 3H-labeled on autoradiography. Thus, the CTL were clearly products of dividing cells, a result that contradicts published data. Reassessment of a previously studied system, which suggested that CTL were not products of cell division, indicated that in that system many of the conjugate-forming cytotoxic cells studied were Ly-2- and nonspecific, and thus perhaps not T cells. We conclude that the clonal selection model is applicable to at least one in vivo T-cell response.
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- Berke G. Enumeration of lymphocyte-target cell conjugates by cytofluorometry. Eur J Immunol. 1985 Apr;15(4):337–340. doi: 10.1002/eji.1830150406. [DOI] [PubMed] [Google Scholar]
- Eichmann K., Fey K., Kuppers R., Melchers I., Simon M. M., Weltzien H. U. Network regulation among T cells; conclusions from limiting dilution experiments. Springer Semin Immunopathol. 1983;6(1):7–32. doi: 10.1007/BF01857364. [DOI] [PubMed] [Google Scholar]
- Kimura A. K., Wigzell H. Development and function of cytotoxic T lymphocytes (CTL). I. In vivo maturation of CTL precursors in the absence of detectable proliferation results as a normal consequence of alloimmunization. J Immunol. 1983 May;130(5):2056–2061. [PubMed] [Google Scholar]
- LIND P. E., BURNET F. M. Recombination between virulent and non-virulent strains of influenza virus. II. The behaviour of virulence markers on recombination. Aust J Exp Biol Med Sci. 1957 Feb;35(1):67–78. doi: 10.1038/icb.1957.8. [DOI] [PubMed] [Google Scholar]
- Mitchison N. A., Pettersson S. Does clonal selection occur among T cells? Ann Immunol (Paris) 1983 Jul-Aug;134D(1):37–45. doi: 10.1016/s0769-2625(83)80054-3. [DOI] [PubMed] [Google Scholar]
- Nossal G. J., Pike B. L. Single cell studies on the antibody-forming potential of fractionated, hapten-specific B lymphocytes. Immunology. 1976 Feb;30(2):189–202. [PMC free article] [PubMed] [Google Scholar]
- Scollay R., Shortman K. Identification of early stages of T lymphocyte development in the thymus cortex and medulla. J Immunol. 1985 Jun;134(6):3632–3642. [PubMed] [Google Scholar]
- Shortman K., Golstein P. Target cell recognition by cytolytic T cells: different requirements for the formation of strong conjugates or for proceeding to lysis. J Immunol. 1979 Aug;123(2):833–839. [PubMed] [Google Scholar]