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. 1986 Aug;83(16):6213–6215. doi: 10.1073/pnas.83.16.6213

Ethanol regulates calcium channels in clonal neural cells.

R O Messing, C L Carpenter, I Diamond, D A Greenberg
PMCID: PMC386470  PMID: 2426713

Abstract

The acute and long-term effects of ethanol on voltage-dependent Ca channel function were studied in PC12, a clonal cell line of neural crest origin. Acute exposure to ethanol produced a concentration-dependent decrease in depolarization-evoked 45Ca2+ uptake, while prolonged (2-10 days) exposure led to a reciprocal increase in 45Ca2+ uptake and in the number of Ca-channel binding sites labeled by the dihydropyridine Ca-channel antagonist [3H]nitrendipine. Uptake was restored to control levels following withdrawal of ethanol from cultures. These findings indicate that cellular adaptation to ethanol may involve enhanced expression of dihydropyridine-sensitive, voltage-dependent Ca channels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bean B. P., Nowycky M. C., Tsien R. W. Beta-adrenergic modulation of calcium channels in frog ventricular heart cells. 1984 Jan 26-Feb 1Nature. 307(5949):371–375. doi: 10.1038/307371a0. [DOI] [PubMed] [Google Scholar]
  2. Brown A. M., Camerer H., Kunze D. L., Lux H. D. Similarity of unitary Ca2+ currents in three different species. Nature. 1982 Sep 9;299(5879):156–158. doi: 10.1038/299156a0. [DOI] [PubMed] [Google Scholar]
  3. COLLIER H. O. A GENERAL THEORY OF THE GENESIS OF DRUG DEPENDENCE BY INDUCTION OF RECEPTORS. Nature. 1965 Jan 9;205:181–182. doi: 10.1038/205181a0. [DOI] [PubMed] [Google Scholar]
  4. Cachelin A. B., de Peyer J. E., Kokubun S., Reuter H. Ca2+ channel modulation by 8-bromocyclic AMP in cultured heart cells. Nature. 1983 Aug 4;304(5925):462–464. doi: 10.1038/304462a0. [DOI] [PubMed] [Google Scholar]
  5. Charness M. E., Gordon A. S., Diamond I. Ethanol modulation of opiate receptors in cultured neural cells. Science. 1983 Dec 16;222(4629):1246–1248. doi: 10.1126/science.6316506. [DOI] [PubMed] [Google Scholar]
  6. Freedman S. B., Dawson G., Villereal M. L., Miller R. J. Identification and characterization of voltage-sensitive calcium channels in neuronal clonal cell lines. J Neurosci. 1984 Jun;4(6):1453–1467. doi: 10.1523/JNEUROSCI.04-06-01453.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Greenberg D. A., Carpenter C. L., Cooper E. C. Stimulation of calcium uptake in PC12 cells by the dihydropyridine agonist BAY K 8644. J Neurochem. 1985 Sep;45(3):990–993. doi: 10.1111/j.1471-4159.1985.tb04095.x. [DOI] [PubMed] [Google Scholar]
  8. Harris R. A., Bruno P. Membrane disordering by anesthetic drugs: relationship to synaptosomal sodium and calcium fluxes. J Neurochem. 1985 Apr;44(4):1274–1281. doi: 10.1111/j.1471-4159.1985.tb08754.x. [DOI] [PubMed] [Google Scholar]
  9. Harris R. A., Hood W. F. Inhibition of synaptosomal calcium uptake by ethanol. J Pharmacol Exp Ther. 1980 Jun;213(3):562–568. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Leslie S. W., Barr E., Chandler J., Farrar R. P. Inhibition of fast- and slow-phase depolarization-dependent synaptosomal calcium uptake by ethanol. J Pharmacol Exp Ther. 1983 Jun;225(3):571–575. [PubMed] [Google Scholar]
  12. Messing R. O., Carpenter C. L., Greenberg D. A. Mechanism of calcium channel inhibition by phenytoin: comparison with classical calcium channel antagonists. J Pharmacol Exp Ther. 1985 Nov;235(2):407–411. [PubMed] [Google Scholar]
  13. Nowycky M. C., Fox A. P., Tsien R. W. Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature. 1985 Aug 1;316(6027):440–443. doi: 10.1038/316440a0. [DOI] [PubMed] [Google Scholar]
  14. Oakes S. G., Pozos R. S. Electrophysiologic effects of acute ethanol exposure. II. Alterations in the calcium component of action potentials from sensory neurons in dissociated culture. Brain Res. 1982 Nov;281(3):251–255. doi: 10.1016/0165-3806(82)90123-7. [DOI] [PubMed] [Google Scholar]
  15. Reuter H. Calcium channel modulation by neurotransmitters, enzymes and drugs. Nature. 1983 Feb 17;301(5901):569–574. doi: 10.1038/301569a0. [DOI] [PubMed] [Google Scholar]
  16. Schmidt J., Rossie S., Catterall W. A. A large intracellular pool of inactive Na channel alpha subunits in developing rat brain. Proc Natl Acad Sci U S A. 1985 Jul;82(14):4847–4851. doi: 10.1073/pnas.82.14.4847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Snyder S. H., Reynolds I. J. Calcium-antagonist drugs. Receptor interactions that clarify therapeutic effects. N Engl J Med. 1985 Oct 17;313(16):995–1002. doi: 10.1056/NEJM198510173131606. [DOI] [PubMed] [Google Scholar]
  18. Stallcup W. B. Sodium and calcium fluxes in a clonal nerve cell line. J Physiol. 1979 Jan;286:525–540. doi: 10.1113/jphysiol.1979.sp012635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stokes J. A., Harris R. A. Alcohols and synaptosomal calcium transport. Mol Pharmacol. 1982 Jul;22(1):99–104. [PubMed] [Google Scholar]
  20. Toll L. Calcium antagonists High-affinity binding and inhibition of calcium transport in a clonal cell line. J Biol Chem. 1982 Nov 25;257(22):13189–13192. [PubMed] [Google Scholar]

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