Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Sep;83(17):6255–6257. doi: 10.1073/pnas.83.17.6255

Peroxidases depolymerize lignin in organic media but not in water

Jonathan S Dordick 1, Michael A Marletta 1, Alexander M Klibanov 1
PMCID: PMC386481  PMID: 16593748

Abstract

Horseradish peroxidase and milk lactoperoxidase, while unable to degrade either synthetic or natural lignins in aqueous solutions, vigorously depolymerize polyconiferyl alcohol, milled wood lignin, and kraft pine lignin in dioxane, dimethylformamide, or methyl formate containing 5% aqueous buffer (10 mM acetate, pH 5). Horseradish peroxidase, solubilized in organic media by chemical modification, can also degrade lignin in native lignocellulose (wheat straw).

Keywords: lignin degradation, biopolymers, enzymes in nonaqueous solvents, ligninase, enzymatic degradation

Full text

PDF
6255

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caldwell E. S., Steelink C. Phenoxy radical intermediates in the enzymatic degradation of lignin model compounds. Biochim Biophys Acta. 1969 Jul 30;184(2):420–431. doi: 10.1016/0304-4165(69)90046-4. [DOI] [PubMed] [Google Scholar]
  2. Freudenberg K. Lignin: Its Constitution and Formation from p-Hydroxycinnamyl Alcohols: Lignin is duplicated by dehydrogenation of these alcohols; intermediates explain formation and structure. Science. 1965 Apr 30;148(3670):595–600. doi: 10.1126/science.148.3670.595. [DOI] [PubMed] [Google Scholar]
  3. Glenn J. K., Morgan M. A., Mayfield M. B., Kuwahara M., Gold M. H. An extracellular H2O2-requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete Phanerochaete chrysosporium. Biochem Biophys Res Commun. 1983 Aug 12;114(3):1077–1083. doi: 10.1016/0006-291x(83)90672-1. [DOI] [PubMed] [Google Scholar]
  4. Kirk T. K., Connors W. J., Bleam R. D., Hackett W. F., Zeikus J. G. Preparation and microbial decomposition of synthetic [14C]ligins. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2515–2519. doi: 10.1073/pnas.72.7.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Tien M., Kirk T. K. Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds. Science. 1983 Aug 12;221(4611):661–663. doi: 10.1126/science.221.4611.661. [DOI] [PubMed] [Google Scholar]
  6. Zaks A., Klibanov A. M. Enzyme-catalyzed processes in organic solvents. Proc Natl Acad Sci U S A. 1985 May;82(10):3192–3196. doi: 10.1073/pnas.82.10.3192. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES