Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Sep;83(17):6263–6266. doi: 10.1073/pnas.83.17.6263

Synthesis of an O-glycosylated cell surface protein induced in yeast by α factor

P Orlean 1,*, H Ammer 1, M Watzele 1, W Tanner 1,†
PMCID: PMC386483  PMID: 16593749

Abstract

A number of cell surface glycoproteins can be specifically and completely released from intact cells of Saccharomyces cerevisiae with 0.5% mercaptoethanol. Among these proteins is one with a molecular mass of 22 kDa, which is synthesized only in haploid a cells treated with the peptide mating pheromone α factor. This protein could be radiolabeled in vivo with [2-3H]mannose, [14C]phenylalanine, and [35S]sulfate. Its synthesis and export to the cell surface were not inhibited by tunicamycin. β-Elimination released almost all radioactivity from the [2-3H]mannose-labeled protein, 36% of its radioactivity being recovered subsequently as mannose and 43% as a dimannoside. Evidence is presented that the 22-kDa O-glycosylated protein is a mating-type specific a cell agglutinin.

Full text

PDF
6263

Images in this article

6264Image
on p.6264
6265Image
on p.6265
6265Image
on p.6265

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boller T., Dürr M., Wiemken A. Characterization of a specific transport system for arginine in isolated yeast vacuoles. Eur J Biochem. 1975 May;54(1):81–91. doi: 10.1111/j.1432-1033.1975.tb04116.x. [DOI] [PubMed] [Google Scholar]
  2. Bücking-Throm E., Duntze W., Hartwell L. H., Manney T. R. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor. Exp Cell Res. 1973 Jan;76(1):99–110. doi: 10.1016/0014-4827(73)90424-2. [DOI] [PubMed] [Google Scholar]
  3. Hasegawa S., Yanagishima N. Alpha-mating-type-specific suppression and a-mating-type-specific enhancement by tunicamycin of sexual agglutinability in the yeast Saccharomyces cerevisiae. Arch Microbiol. 1984 Aug;138(4):310–314. doi: 10.1007/BF00410896. [DOI] [PubMed] [Google Scholar]
  4. Kidby D. K., Davies R. Invertase and disulphide bridges in the yeast wall. J Gen Microbiol. 1970 Jun;61(3):327–333. doi: 10.1099/00221287-61-3-327. [DOI] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Lipke P. N., Ballou C. E. Altered immunochemical reactivity of Saccharomyces cerevisiae a-cells after alpha-factor-induced morphogenesis. J Bacteriol. 1980 Mar;141(3):1170–1177. doi: 10.1128/jb.141.3.1170-1177.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lipke P. N., Taylor A., Ballou C. E. Morphogenic effects of alpha-factor on Saccharomyces cerevisiae a cells. J Bacteriol. 1976 Jul;127(1):610–618. doi: 10.1128/jb.127.1.610-618.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Orlean P., Arnold E., Tanner W. Apparent inhibition of glycoprotein synthesis by S.cerevisiae mating pheromones. FEBS Lett. 1985 May 20;184(2):313–317. doi: 10.1016/0014-5793(85)80629-3. [DOI] [PubMed] [Google Scholar]
  9. Pierce M., Ballou C. E. Cell-cell recognition in yeast. Characterization of the sexual agglutination factors from Saccharomyces kluyveri. J Biol Chem. 1983 Mar 25;258(6):3576–3582. [PubMed] [Google Scholar]
  10. Schekman R., Brawley V. Localized deposition of chitin on the yeast cell surface in response to mating pheromone. Proc Natl Acad Sci U S A. 1979 Feb;76(2):645–649. doi: 10.1073/pnas.76.2.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sprague G. F., Jr, Blair L. C., Thorner J. Cell interactions and regulation of cell type in the yeast Saccharomyces cerevisiae. Annu Rev Microbiol. 1983;37:623–660. doi: 10.1146/annurev.mi.37.100183.003203. [DOI] [PubMed] [Google Scholar]
  12. Sprague G. F., Jr, Herskowitz I. Control of yeast cell type by the mating type locus. I. Identification and control of expression of the a-specific gene BAR1. J Mol Biol. 1981 Dec 5;153(2):305–321. doi: 10.1016/0022-2836(81)90280-1. [DOI] [PubMed] [Google Scholar]
  13. Stetler G. L., Thorner J. Molecular cloning of hormone-responsive genes from the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1144–1148. doi: 10.1073/pnas.81.4.1144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weimberg R., Orton W. L. Elution of exocellular enzymes from Saccharomyces fragilis and Saccharomyces cerevisiae. J Bacteriol. 1966 Jan;91(1):1–13. doi: 10.1128/jb.91.1.1-13.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yen P. H., Ballou C. E. Partial characterization of the sexual agglutination factor from Hansenula wingei Y-2340 type 5 cells. Biochemistry. 1974 May 21;13(11):2428–2437. doi: 10.1021/bi00708a030. [DOI] [PubMed] [Google Scholar]
  16. Yoshida K., HAGIYA M., Yanagishima N. Isolation and purification of the sexual agglutination substance of mating type a cells in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1976 Aug 23;71(4):1085–1094. doi: 10.1016/0006-291x(76)90765-8. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES