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. 1996 Aug 6;93(16):8247–8252. doi: 10.1073/pnas.93.16.8247

Sequence and expression of the murine Hoxd-3 homeobox gene.

D P Tan 1, X Shao 1, L Pu 1, V Guo 1, M Nirenberg 1
PMCID: PMC38655  PMID: 8710855

Abstract

Murine Hoxd-3 (Hox 4.1) genomic DNA and cDNA and Hoxa-3 (Hox 1.5) cDNA were cloned and sequenced. The homeodomains of Hoxd-3 and Hoxa-3 and regions before and after the homeodomain are highly conserved. Both Hoxa-3 and Hoxa-3 proteins have a proline-rich region that contains consensus amino acid sequences for binding to Src homology 3 domains of some signal transduction proteins. Northern blot analysis of RNA from 8- to 11-day-old mouse embryos revealed a 4.3-kb species of Hoxd-3 RNA, whereas a less abundant 3.0-kb species of Hoxd-3 RNA was found in RNA from 9- to 11-day-old embryos. Two species of Hoxd-3 poly(A)+ RNA, 4.3 and 6.0 kb in length, were found in poly(A)+ RNA from adult mouse kidney, but not in RNA from other adult tissues tested. Hoxd-3 mRNA was detected by in situ hybridization in 12-, 14-, and 17-day-old mouse embryos in the posterior half of the myelencephalon, spinal cord, dorsal root ganglia, first cervical vertebra, thyroid gland, kidney tubules, esophagus, stomach, and intestines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arcioni L., Simeone A., Guazzi S., Zappavigna V., Boncinelli E., Mavilio F. The upstream region of the human homeobox gene HOX3D is a target for regulation by retinoic acid and HOX homeoproteins. EMBO J. 1992 Jan;11(1):265–277. doi: 10.1002/j.1460-2075.1992.tb05049.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chisaka O., Capecchi M. R. Regionally restricted developmental defects resulting from targeted disruption of the mouse homeobox gene hox-1.5. Nature. 1991 Apr 11;350(6318):473–479. doi: 10.1038/350473a0. [DOI] [PubMed] [Google Scholar]
  3. Condie B. G., Capecchi M. R. Mice homozygous for a targeted disruption of Hoxd-3 (Hox-4.1) exhibit anterior transformations of the first and second cervical vertebrae, the atlas and the axis. Development. 1993 Nov;119(3):579–595. doi: 10.1242/dev.119.3.579. [DOI] [PubMed] [Google Scholar]
  4. Condie B. G., Capecchi M. R. Mice with targeted disruptions in the paralogous genes hoxa-3 and hoxd-3 reveal synergistic interactions. Nature. 1994 Jul 28;370(6487):304–307. doi: 10.1038/370304a0. [DOI] [PubMed] [Google Scholar]
  5. Gout I., Dhand R., Hiles I. D., Fry M. J., Panayotou G., Das P., Truong O., Totty N. F., Hsuan J., Booker G. W. The GTPase dynamin binds to and is activated by a subset of SH3 domains. Cell. 1993 Oct 8;75(1):25–36. [PubMed] [Google Scholar]
  6. Hunt P., Gulisano M., Cook M., Sham M. H., Faiella A., Wilkinson D., Boncinelli E., Krumlauf R. A distinct Hox code for the branchial region of the vertebrate head. Nature. 1991 Oct 31;353(6347):861–864. doi: 10.1038/353861a0. [DOI] [PubMed] [Google Scholar]
  7. Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125–8148. doi: 10.1093/nar/15.20.8125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LaRosa G. J., Gudas L. J. Early retinoic acid-induced F9 teratocarcinoma stem cell gene ERA-1: alternate splicing creates transcripts for a homeobox-containing protein and one lacking the homeobox. Mol Cell Biol. 1988 Sep;8(9):3906–3917. doi: 10.1128/mcb.8.9.3906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lonai P., Arman E., Czosnek H., Ruddle F. H., Blatt C. New murine homeoboxes: structure, chromosomal assignment, and differential expression in adult erythropoiesis. DNA. 1987 Oct;6(5):409–418. doi: 10.1089/dna.1987.6.409. [DOI] [PubMed] [Google Scholar]
  10. McGinnis W., Hart C. P., Gehring W. J., Ruddle F. H. Molecular cloning and chromosome mapping of a mouse DNA sequence homologous to homeotic genes of Drosophila. Cell. 1984 Oct;38(3):675–680. doi: 10.1016/0092-8674(84)90262-9. [DOI] [PubMed] [Google Scholar]
  11. McGinnis W., Krumlauf R. Homeobox genes and axial patterning. Cell. 1992 Jan 24;68(2):283–302. doi: 10.1016/0092-8674(92)90471-n. [DOI] [PubMed] [Google Scholar]
  12. Pravtcheva D., Newman M., Hunihan L., Lonai P., Ruddle F. H. Chromosome assignment of the murine Hox-4.1 gene. Genomics. 1989 Oct;5(3):541–545. doi: 10.1016/0888-7543(89)90021-9. [DOI] [PubMed] [Google Scholar]
  13. Ren R., Mayer B. J., Cicchetti P., Baltimore D. Identification of a ten-amino acid proline-rich SH3 binding site. Science. 1993 Feb 19;259(5098):1157–1161. doi: 10.1126/science.8438166. [DOI] [PubMed] [Google Scholar]
  14. Rijli F. M., Matyas R., Pellegrini M., Dierich A., Gruss P., Dollé P., Chambon P. Cryptorchidism and homeotic transformations of spinal nerves and vertebrae in Hoxa-10 mutant mice. Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8185–8189. doi: 10.1073/pnas.92.18.8185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sham M. H., Hunt P., Nonchev S., Papalopulu N., Graham A., Boncinelli E., Krumlauf R. Analysis of the murine Hox-2.7 gene: conserved alternative transcripts with differential distributions in the nervous system and the potential for shared regulatory regions. EMBO J. 1992 May;11(5):1825–1836. doi: 10.1002/j.1460-2075.1992.tb05234.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stubbs L., Poustka A., Baron A., Lehrach H., Lonai P., Duboule D. The murine genes Hox-5.1 and Hox-4.1 belong to the same HOX complex on chromosome 2. Genomics. 1990 Jul;7(3):422–427. doi: 10.1016/0888-7543(90)90177-v. [DOI] [PubMed] [Google Scholar]
  18. Taniguchi Y., Fujii A., Moriuchi T. Cloning and sequencing of the human homeobox gene HOX4A. Biochim Biophys Acta. 1992 Oct 20;1132(3):332–334. doi: 10.1016/0167-4781(92)90173-w. [DOI] [PubMed] [Google Scholar]

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