Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Sep;83(17):6618–6621. doi: 10.1073/pnas.83.17.6618

Cloning and DNA sequence analysis of the cDNA for the precursor of the beta chain of bovine follicle stimulating hormone.

F S Esch, A J Mason, K Cooksey, M Mercado, S Shimasaki
PMCID: PMC386555  PMID: 3092216

Abstract

Follicle stimulating hormone (FSH) plays essential roles in the maintenance and development of oocytes and spermatozoa in normal reproductive physiology. FSH possesses two subunits, alpha and beta, the latter being responsible for FSH biological specificity. We have cloned and sequenced the cDNA encoding the FSH beta chain from a bovine anterior pituitary cDNA library. The mature molecule is 109 amino acids long and is preceded by a 20-amino acid putative signal peptide. RNA gel blot analysis of bovine pituitary RNA shows that the mRNA encoding beta chain of FSH is approximately 1.7 kilobases in length.

Full text

PDF

Images in this article

6619Image
on p.6619

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agarwal K. L., Brunstedt J., Noyes B. E. A general method for detection and characterization of an mRNA using an oligonucleotide probe. J Biol Chem. 1981 Jan 25;256(2):1023–1028. [PubMed] [Google Scholar]
  2. Chin W. W., Godine J. E., Klein D. R., Chang A. S., Tan L. K., Habener J. F. Nucleotide sequence of the cDNA encoding the precursor of the beta subunit of rat lutropin. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4649–4653. doi: 10.1073/pnas.80.15.4649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chin W. W., Muccini J. A., Jr, Shin L. Evidence for a single rat thyrotropin-beta-subunit gene: thyroidectomy increases its mRNA. Biochem Biophys Res Commun. 1985 May 16;128(3):1152–1158. doi: 10.1016/0006-291x(85)91061-7. [DOI] [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Closset J., Maghuin-Rogister G., Hennen G., Strosberg A. D. Porcine follitropin. The amino-acid sequence of the beta subunit. Eur J Biochem. 1978 May;86(1):115–120. doi: 10.1111/j.1432-1033.1978.tb12290.x. [DOI] [PubMed] [Google Scholar]
  6. Dobner P. R., Kawasaki E. S., Yu L. Y., Bancroft F. C. Thyroid or glucocorticoid hormone induces pre-growth-hormone mRNA and its probable nuclear precursor in rat pituitary cells. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2230–2234. doi: 10.1073/pnas.78.4.2230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fiddes J. C., Goodman H. M. The cDNA for the beta-subunit of human chorionic gonadotropin suggests evolution of a gene by readthrough into the 3'-untranslated region. Nature. 1980 Aug 14;286(5774):684–687. doi: 10.1038/286684a0. [DOI] [PubMed] [Google Scholar]
  8. Fiddes J. C., Goodman H. M. The gene encoding the common alpha subunit of the four human glycoprotein hormones. J Mol Appl Genet. 1981;1(1):3–18. [PubMed] [Google Scholar]
  9. Fiddes J. C., Talmadge K. Structure, expression, and evolution of the genes for the human glycoprotein hormones. Recent Prog Horm Res. 1984;40:43–78. doi: 10.1016/b978-0-12-571140-1.50006-2. [DOI] [PubMed] [Google Scholar]
  10. Fujiki Y., Rathnam P., Saxena B. B. Amino acid sequence of the beta-subunit of the follicle-stimulating hormone from equine pituitary glands. J Biol Chem. 1978 Aug 10;253(15):5363–5368. [PubMed] [Google Scholar]
  11. Grantham R., Gautier C., Gouy M., Jacobzone M., Mercier R. Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res. 1981 Jan 10;9(1):r43–r74. doi: 10.1093/nar/9.1.213-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gurr J. A., Catterall J. F., Kourides I. A. Cloning of cDNA encoding the pre-beta subunit of mouse thyrotropin. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2122–2126. doi: 10.1073/pnas.80.8.2122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Habener J. F., Rosenblatt M., Kemper B., Kronenberg H. M., Rich A., Potts J. T., Jr Pre-proparathyroid hormone; amino acid sequence, chemical synthesis, and some biological studies of the precursor region. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2616–2620. doi: 10.1073/pnas.75.6.2616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kozak M. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 1981 Oct 24;9(20):5233–5252. doi: 10.1093/nar/9.20.5233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kreil G. Transfer of proteins across membranes. Annu Rev Biochem. 1981;50:317–348. doi: 10.1146/annurev.bi.50.070181.001533. [DOI] [PubMed] [Google Scholar]
  16. Maurer R. A., Croyle M. L., Donelson J. E. The sequence of a cloned cDNA for the beta subunit of bovine thyrotropin predicts a protein containing both NH2-and COOH-terminal extensions. J Biol Chem. 1984 Apr 25;259(8):5024–5027. [PubMed] [Google Scholar]
  17. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  18. Sairam M. R., Seidah N. G., Chrétien M. Primary structure of the ovine pituitary follitropin beta-subunit. Biochem J. 1981 Sep 1;197(3):541–552. doi: 10.1042/bj1970541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Saxena B. B., Rathnam P. Amino acid sequence of the beta subunit of follicle-stimulating hormone from human pituitary glands. J Biol Chem. 1976 Feb 25;251(4):993–1005. [PubMed] [Google Scholar]
  21. Setzer D. R., McGrogan M., Nunberg J. H., Schimke R. T. Size heterogeneity in the 3' end of dihydrofolate reductase messenger RNAs in mouse cells. Cell. 1980 Nov;22(2 Pt 2):361–370. doi: 10.1016/0092-8674(80)90346-3. [DOI] [PubMed] [Google Scholar]
  22. Talmadge K., Boorstein W. R., Fiddes J. C. The human genome contains seven genes for the beta-subunit of chorionic gonadotropin but only one gene for the beta-subunit of luteinizing hormone. DNA. 1983;2(4):281–289. doi: 10.1089/dna.1983.2.281. [DOI] [PubMed] [Google Scholar]
  23. Tepper M. A., Roberts J. L. Evidence for only one beta-luteinizing hormone and no beta-chorionic gonadotropin gene in the rat. Endocrinology. 1984 Jul;115(1):385–391. doi: 10.1210/endo-115-1-385. [DOI] [PubMed] [Google Scholar]
  24. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tosi M., Young R. A., Hagenbüchle O., Schibler U. Multiple polyadenylation sites in a mouse alpha-amylase gene. Nucleic Acids Res. 1981 May 25;9(10):2313–2323. doi: 10.1093/nar/9.10.2313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Ullrich A., Berman C. H., Dull T. J., Gray A., Lee J. M. Isolation of the human insulin-like growth factor I gene using a single synthetic DNA probe. EMBO J. 1984 Feb;3(2):361–364. doi: 10.1002/j.1460-2075.1984.tb01812.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES