Abstract
We have developed a simple, objectively scorable test for the mutagenicity of chemical compounds which can be fed Drosophila melanogaster. The test depends upon the somatic reversion of the X chromosome, recessive eye color mutation, white-ivory (wi) to wild type (w+). Reversions are scored as clones of w+ facets in the wi eyes of eclosing adults. To increase the sensitivity, a tandem quadruplication containing four wi mutations was synthesized. Thus, in homozygous females eight wi mutations are potentially revertible. Six mutagenic compounds, all alkylating agents, all gave positive results at several concentrations tested. Molecular analysis demonstrates that the induced reversions, germinal and somatic, are associated with the loss of 2.9-kilobase DNA duplicated in the wi mutation.
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- Bowman J. T., Jr, Green M. M. X-ray-induced reversion of the white-ivory mutant of Drosophila melanogaster. Genetica. 1966;37(1):7–16. doi: 10.1007/BF01547115. [DOI] [PubMed] [Google Scholar]
- Bowman J. T., Jr Spontaneous reversion of the white-ivory mutant of Drosophila melanogaster. Genetics. 1965 Nov;52(5):1069–1079. doi: 10.1093/genetics/52.5.1069. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bowman J. T. Parameters of spontaneous and x-ray-induced reversion of the white-ivory mutant of Drosophila. Mutat Res. 1969 May-Jun;7(3):409–415. doi: 10.1016/0027-5107(69)90111-0. [DOI] [PubMed] [Google Scholar]
- Karess R. E., Rubin G. M. A small tandem duplication is responsible for the unstable white-ivory mutation in Drosophila. Cell. 1982 Aug;30(1):63–69. doi: 10.1016/0092-8674(82)90012-5. [DOI] [PubMed] [Google Scholar]
- Kuner J. M., Nakanishi M., Ali Z., Drees B., Gustavson E., Theis J., Kauvar L., Kornberg T., O'Farrell P. H. Molecular cloning of engrailed: a gene involved in the development of pattern in Drosophila melanogaster. Cell. 1985 Aug;42(1):309–316. doi: 10.1016/s0092-8674(85)80126-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUERS H. Untersuchung über die Mutagenität des Triäthylenmelamin (TEM) an Drosophila melanogaster. Arch Geschwulstforsch. 1953;6(1):77–83. [PubMed] [Google Scholar]
- Levis R., Bingham P. M., Rubin G. M. Physical map of the white locus of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Jan;79(2):564–568. doi: 10.1073/pnas.79.2.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maron D. M., Ames B. N. Revised methods for the Salmonella mutagenicity test. Mutat Res. 1983 May;113(3-4):173–215. doi: 10.1016/0165-1161(83)90010-9. [DOI] [PubMed] [Google Scholar]
- McCann J., Choi E., Yamasaki E., Ames B. N. Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5135–5139. doi: 10.1073/pnas.72.12.5135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakoyama Y., Todo T., Ishiwa-Chigusa S., Honjo T., Kondo S. Structures of defective P transposable elements prevalent in natural Q and Q-derived M strains of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6236–6239. doi: 10.1073/pnas.82.18.6236. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]