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. 1986 Sep;83(18):6825–6828. doi: 10.1073/pnas.83.18.6825

Cloning and nitrate induction of nitrate reductase mRNA

Chi-Lien Cheng *, Julia Dewdney *, Andris Kleinhofs †, Howard M Goodman *,‡
PMCID: PMC386602  PMID: 16593758

Abstract

Nitrate is the major source of nitrogen taken from the soil by higher plants but requires reduction to ammonia prior to incorporation into amino acids. The first enzyme in the reducing pathway is a nitrate-inducible enzyme, nitrate reductase (EC 1.6.6.1). A specific polyclonal antiserum raised against purified barley nitrate reductase has been used to immunoprecipitate in vivo labeled protein and in vitro translation products, demonstrating that nitrate induction increases nitrate reductase protein and translatable mRNA. A partial cDNA clone for barley nitrate reductase has been isolated and identified by hybrid-selected translation. RNA blot-hybridization analysis shows that nitrate induction also causes a marked increase in the steady-state level of nitrate reductase mRNA.

Keywords: barley, gel separation, hybrid-selected translation

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Selected References

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