Fig. 1.

SP2012 and SP5031 block endothelial cell adhesion alone and synergistically in combination. a Sequences of selected peptides: SP2012, a collagen IV mimetic peptide and SP5031, a somatotropin-domain derived peptide. b SP2012 and SP5031 both block endothelial cell adhesion on cell-culture treated plates (no extracellular matrix coating) and show synergy. SD of the mean (±) is shown. c Graphical representation of combinatorial dosing. All dosing combinations show synergy as determined by the Chou-Talalay method. d SP2012 and SP5031 synergistically block LEC adhesion on cell-culture treated plates. e Graphical representation of peptide doses on LECs. f Isobologram showing experimental values demonstrating synergy. g Different extracellular matrices shown including fibronectin, gelatin, and laminin coatings. SP2012 and SP5031 synergistically block cell adhesion on uncoated plates, fibronectin, and gelatin-coated plates. SP5031 does not block cell attachment to laminin at 5 µM