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. 1986 Oct;83(19):7429–7433. doi: 10.1073/pnas.83.19.7429

Isolation and characterization of a primitive interleukin-1-like protein from an invertebrate, Asterias forbesi.

G Beck, G S Habicht
PMCID: PMC386731  PMID: 3489938

Abstract

Interleukin 1 (IL-1) is the major immunoregulatory molecule produced by macrophages and other related cells. It acts as the molecular orchestrator of nonspecific host defense mechanisms against multifarious insults. In this study we report the characterization of an IL-1-like protein from the starfish Asterias forbesi. IL-1-like activity was found in the coelomocytes of this invertebrate and was also isolated and purified from the coelomic fluid. The coelomocytes were capable of phagocytosing latex particles and were adherent to plastic tissue culture dishes. Starfish IL-1 stimulated murine thymocyte proliferation directly and to a greater degree in the presence of submitogenic concentrations of concanavalin A. Starfish IL-1 also stimulated fibroblast proliferation and fibroblast protein synthesis. It was found to have a Mr of 29,500 and pI values of 7.5, 5.9, and 4.8. Starfish IL-1 activity was inhibited by an antibody to human IL-1. These characteristics suggest starfish IL-1 to be very similar to murine and human IL-1. The similarities to vertebrate IL-1 considered along with the evolutionary position of the starfish suggest that IL-1 is an important, ancient, and functionally conserved molecule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auron P. E., Webb A. C., Rosenwasser L. J., Mucci S. F., Rich A., Wolff S. M., Dinarello C. A. Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7907–7911. doi: 10.1073/pnas.81.24.7907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dinarello C. A., Goldin N. P., Wolff S. M. Demonstration and characterization of two distinct human leukocytic pyrogens. J Exp Med. 1974 Jun 1;139(6):1369–1381. doi: 10.1084/jem.139.6.1369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dinarello C. A. Interleukin-1. Rev Infect Dis. 1984 Jan-Feb;6(1):51–95. doi: 10.1093/clinids/6.1.51. [DOI] [PubMed] [Google Scholar]
  4. Habicht G. S., Beck G., Benach J. L., Coleman J. L., Leichtling K. D. Lyme disease spirochetes induce human and murine interleukin 1 production. J Immunol. 1985 May;134(5):3147–3154. [PubMed] [Google Scholar]
  5. Ihrie E. J., Wood D. D. Biochemical heterogeneity of human interleukin-1. Lymphokine Res. 1985 Summer;4(3):169–181. [PubMed] [Google Scholar]
  6. Kampschmidt R. F. The numerous postulated biological manifestations of interleukin-1. J Leukoc Biol. 1984 Sep;36(3):341–355. doi: 10.1002/jlb.36.3.341. [DOI] [PubMed] [Google Scholar]
  7. Lomedico P. T., Gubler U., Hellmann C. P., Dukovich M., Giri J. G., Pan Y. C., Collier K., Semionow R., Chua A. O., Mizel S. B. Cloning and expression of murine interleukin-1 cDNA in Escherichia coli. 1984 Nov 29-Dec 5Nature. 312(5993):458–462. doi: 10.1038/312458a0. [DOI] [PubMed] [Google Scholar]
  8. McKernan L. N., Largen M. T. Identification of multiple-molecular-weight forms of thymocyte comitogenic activity from the monocyte/macrophage cell line RAW 264.7. Cell Immunol. 1983 Aug;80(1):84–96. doi: 10.1016/0008-8749(83)90096-5. [DOI] [PubMed] [Google Scholar]
  9. Mizel S. B. Physicochemical characterization of lymphocyte-activating factor (LAF). J Immunol. 1979 Jun;122(6):2167–2172. [PubMed] [Google Scholar]
  10. Mizel S. B., Rosenstreich D. L. Regulation of lymphocyte-activating factor (LAF) production and secretion in P388D1 cells: identification of high molecular weight precursors of LAF. J Immunol. 1979 Jun;122(6):2173–2179. [PubMed] [Google Scholar]
  11. Mizel S. B. Studies on the purification and structure-functional relationships of murine lymphocyte activating factor (Interleukin 1). Mol Immunol. 1980 May;17(5):571–577. doi: 10.1016/0161-5890(80)90155-8. [DOI] [PubMed] [Google Scholar]
  12. Postlethwaite A. E., Lachman L. B., Kang A. H. Induction of fibroblast proliferation by interleukin-1 derived from human monocytic leukemia cells. Arthritis Rheum. 1984 Sep;27(9):995–1001. doi: 10.1002/art.1780270905. [DOI] [PubMed] [Google Scholar]
  13. Prendergast R. A., Cole G. A., Henney C. S. Marine invertebrate origin of a reactant to mammalian T cells. Ann N Y Acad Sci. 1974;234(0):7–17. doi: 10.1111/j.1749-6632.1974.tb53014.x. [DOI] [PubMed] [Google Scholar]
  14. Prendergast R. A., Suzuki M. Invertebrate protein simulating mediators of delayed hypersensitivity. Nature. 1970 Jul 18;227(5255):277–279. doi: 10.1038/227277a0. [DOI] [PubMed] [Google Scholar]
  15. Quigley J. P., Armstrong P. B. An endopeptidase inhibitor, similar to mammalian alpha 2-macroglobulin, detected in the hemolymph of an invertebrate, Limulus polyphemus. J Biol Chem. 1983 Jul 10;258(13):7903–7906. [PubMed] [Google Scholar]
  16. Rosenwasser L. J., Dinarello C. A. Ability of human leukocytic pyrogen to enhance phytohemagglutinin induced murine thymocyte proliferation. Cell Immunol. 1981 Sep 1;63(1):134–142. doi: 10.1016/0008-8749(81)90034-4. [DOI] [PubMed] [Google Scholar]
  17. Schmidt J. A., Mizel S. B., Cohen D., Green I. Interleukin 1, a potential regulator of fibroblast proliferation. J Immunol. 1982 May;128(5):2177–2182. [PubMed] [Google Scholar]
  18. Tschesche H., Dietl T. Trypsin-kallikrein inhibitors from snails (Helix pomatia). Methods Enzymol. 1976;45:772–785. doi: 10.1016/s0076-6879(76)45070-x. [DOI] [PubMed] [Google Scholar]
  19. Willenborg D. O., Prendergast R. A. The effect of sea star coelomocyte extract on cell-mediated resistance to Listeria monocytogenes in mice. J Exp Med. 1974 Apr 1;139(4):820–833. doi: 10.1084/jem.139.4.820. [DOI] [PMC free article] [PubMed] [Google Scholar]

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