Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Oct;83(19):7561–7564. doi: 10.1073/pnas.83.19.7561

Developmental aspects of proline transport in rat renal brush border membranes.

M S Medow, K S Roth, D R Goldmann, K Ginkinger, B Y Hsu, S Segal
PMCID: PMC386759  PMID: 3463985

Abstract

Proline uptake by rat renal brush border membrane vesicles from animals 7 days of age and older has been examined to delineate developmental changes in membrane function that may underlie the physiological hyperprolinuria of young animals. Although the two proline transport systems normally present in adult membranes were found in membranes from young animals, the proline "overshoot" resulting from a sodium ion gradient is minimal and increases with age of the animal from which the membranes were isolated. This is associated with a severalfold faster entry of 22Na into vesicles of the 7-day-old animal compared to entry into membranes prepared from adult kidneys. The very rapid dissipation of the sodium gradient thus diminishing the driving force for transmembrane proline movement may explain the changes in proline overshoot observed in membranes from young animals. The altered sodium permeability is consistent with the fact that young animals have a generalized inability to reabsorb other amino acids whose transport is known to be sodium gradient stimulated.

Full text

PDF
7561

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baerlocher K. E., Scriver C. R., Mohyuddin F. Ontogeny of iminoglycine transport in mammalian kidney. Proc Natl Acad Sci U S A. 1970 Apr;65(4):1009–1016. doi: 10.1073/pnas.65.4.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baerlocher K. E., Scriver C. R., Mohyuddin F. The ontogeny of amino acid transport in rat kidney. I. Effect on distribution ratios and intracellular metabolism of proline and glycine. Biochim Biophys Acta. 1971 Dec 3;249(2):353–363. doi: 10.1016/0005-2736(71)90114-3. [DOI] [PubMed] [Google Scholar]
  3. Baerlocher K. E., Scriver C. R., Mohyuddin F. The ontogeny of amino acid transport in rat kidney. II. Kinetics of uptake and effect of anoxia. Biochim Biophys Acta. 1971 Dec 3;249(2):364–372. doi: 10.1016/0005-2736(71)90115-5. [DOI] [PubMed] [Google Scholar]
  4. Blazer-Yost B., Jezyk P. F. Free amino acids in the plasma and urine of dogs from birth to senescence. Am J Vet Res. 1979 Jun;40(6):832–838. [PubMed] [Google Scholar]
  5. Foreman J. W., Medow M. S., Wald H., Ginkinger K., Segal S. Developmental aspects of sugar transport by isolated dog renal cortical tubules. Pediatr Res. 1984 Aug;18(8):719–723. doi: 10.1203/00006450-198408000-00008. [DOI] [PubMed] [Google Scholar]
  6. Ghishan F. K., Wilson F. A. Developmental maturation of D-glucose transport by rat jejunal brush-border membrane vesicles. Am J Physiol. 1985 Jan;248(1 Pt 1):G87–G92. doi: 10.1152/ajpgi.1985.248.1.G87. [DOI] [PubMed] [Google Scholar]
  7. Goldmann D. R., Schlesinger H., Segal S. Isolation and characterization of the brush border fraction from newborn rat renal proximal tubule cells. Biochim Biophys Acta. 1976 Jan 21;419(2):251–260. doi: 10.1016/0005-2736(76)90351-5. [DOI] [PubMed] [Google Scholar]
  8. Hsu B. Y., Corcoran S. M., Marshall C. M., Segal S. The effect of papain upon proline and sodium transport of rat renal brush-border membrane vesicles. Biochim Biophys Acta. 1983 Oct 26;735(1):40–52. doi: 10.1016/0005-2736(83)90259-6. [DOI] [PubMed] [Google Scholar]
  9. Hwang S. M., Serabian M. A., Roth K. S., Segal S. L-Proline transport by isolated renal tubules from newborn and adult rats. Pediatr Res. 1983 Jan;17(1):42–46. doi: 10.1203/00006450-198301000-00008. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. McNamara P. D., Ozegović B., Pepe L. M., Segal S. Proline and glycine uptake by renal brushborder membrane vesicles. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4521–4525. doi: 10.1073/pnas.73.12.4521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Medow M. S., Foreman J. W., Bovee K. C., Segal S. Developmental changes of glycine transport in the dog. Biochim Biophys Acta. 1982 Dec 8;693(1):85–92. doi: 10.1016/0005-2736(82)90473-4. [DOI] [PubMed] [Google Scholar]
  13. Medow M. S., Reynolds R., Bovee K. C., Segal S. Proline and glucose transport by renal membranes from dogs with spontaneous idiopathic Fanconi syndrome. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7769–7772. doi: 10.1073/pnas.78.12.7769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Medow M. S., Roth K. S., Ginkinger K., Segal S. Renal brush-border-membrane vesicles prepared from newborn rats by free-flow electrophoresis and their proline uptake. Biochem J. 1983 Jul 15;214(1):209–214. doi: 10.1042/bj2140209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pockrandt-Hemstedt H., Schmitz J. E., Kinne-Saffran E., Kinne R. Morphologische und biochemische Untersuchungen über die Oberflächenstruktur der Bürstensaummenbran der Rattenniere. Pflugers Arch. 1972;333(4):297–313. doi: 10.1007/BF00586210. [DOI] [PubMed] [Google Scholar]
  16. Rosenberg L. E., Segal S. Maleic acid-induced inhibition of amino acid transport in rat kidney. Biochem J. 1964 Aug;92(2):345–352. doi: 10.1042/bj0920345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roth K. S., Hwang S. M., London J. W., Segal S. Ontogeny of glycine transport in isolated rat renal tubules. Am J Physiol. 1977 Sep;233(3):F241–F246. doi: 10.1152/ajprenal.1977.233.3.F241. [DOI] [PubMed] [Google Scholar]
  18. Scriver C. R., Davies E. Endogenous renal clearance rates of free amino acids in pre-pubertal children. (Employing an accelerated procedure for elution chromatography of basic amino acids on ion exchange resin). Pediatrics. 1965 Oct;36(4):592–598. [PubMed] [Google Scholar]
  19. Segal S., Rea C., Smith I. Separate transport systems for sugars and amino acids in developing rat kidney cortex. Proc Natl Acad Sci U S A. 1971 Feb;68(2):372–376. doi: 10.1073/pnas.68.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Webber W. A., Cairns J. A. A comparison of the amino acid concentrating ability of the kidney cortex of newborn and mature rats. Can J Physiol Pharmacol. 1968 Mar;46(2):165–169. doi: 10.1139/y68-027. [DOI] [PubMed] [Google Scholar]
  21. Weiss S. D., McNamara P. D., Segal S. Uptake of proline by renal brushborder vesicles: a mathematical analysis. J Theor Biol. 1981 Dec 7;93(3):597–608. doi: 10.1016/0022-5193(81)90223-x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES