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. 1986 Oct;83(20):7821–7825. doi: 10.1073/pnas.83.20.7821

Toward a physical map of the genome of the nematode Caenorhabditis elegans

Alan Coulson 1, John Sulston 1, Sydney Brenner 1, Jonathan Karn 1
PMCID: PMC386814  PMID: 16593771

Abstract

A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.

Keywords: ordered clone bank, genomic data base, clone matching

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Selected References

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