Abstract
Propionibacterium acnes is a Gram-positive bacterium that is closely associated with various parts of the human body, in particular with sebaceous follicles of the skin. It has also been frequently isolated from diseased human prostates. Here, we report draft genome sequences of two P. acnes strains, P6 and PA2, isolated from radical prostatectomy specimens.
GENOME ANNOUNCEMENT
Propionibacterium acnes is closely associated with humans as a prominent member of the skin microbiota. P. acnes has been strongly linked to acne vulgaris, a common skin disease that affects mainly adolescents. In addition, the bacterium is suspected to be associated with other nonskin diseases, including sarcoidosis and prostate pathologies (1–3). The bacterium has been detected in cancerous prostates and was cultivated from radical prostatectomy specimens (2–4). By use of a multilocus sequence typing (MLST) approach (5), the P. acnes prostate isolates were classified predominantly as type I-2 and type II strains (4). These P. acnes subtypes occur less frequently on human skin than do type IA strains (5). Here, we describe the draft genome sequences of two type I-2 strains, P6 and PA2. The P6 strain was isolated from a radical prostatectomy specimen obtained at the Charité Hospital in Berlin, Germany (3), and the PA2 strain was isolated at the Johns Hopkins Hospital, Baltimore, MD, from a radical prostatectomy specimen that contained both acute and chronic inflammation (6).
Genomic DNA of P. acnes was isolated using the MasterPure Gram-positive DNA purification kit (Epicentre). A genomic library was constructed and subjected to paired-end sequencing using a HiSeq Illumina sequencer at the Beijing Genomics Institute (Shenzhen, China). The assembly of sequence reads was done using SOAPdenovo (version 1.05); it resulted in draft chromosomes of 2,535,516 bp (32 contigs) and 2,526,264 bp (138 contigs) for strains P6 and PA2, respectively. The GC content was 60% for both draft genomes. By use of the Prokaryotic Genome Automatic Annotation Pipeline (PGAAP) of NCBI, 2,332 and 2,391 coding sequences (CDS) in strains P6 and PA2, respectively, were predicted.
The type I-2 P. acnes strains P6 and PA2 were recently used for in vitro and in vivo prostate infection models, respectively (3, 6, 7). It was revealed that infections with strains P6 and PA2 led to induction of inflammation-related genes in a cell line model and long-term chronic inflammation in a mouse prostate model, respectively. Whether P. acnes is involved in human prostate cancer development and/or progression remains an open future research question. The genome information for prostate isolates of P. acnes could aid our understanding of the potential role of P. acnes in prostate-related diseases.
Nucleotide sequence accession numbers.
The draft genome sequences were deposited in the DDBJ/EMBL/GenBank database under the accession numbers ARZZ00000000 (P. acnes P6) and APCV00000000 (P. acnes PA2).
ACKNOWLEDGMENTS
This work was funded in part by the Danish Council for Independent Research (to H.B.) and the Department of Defense Prostate Cancer Research Program award W81XWH-11-1-0521 (to K.S.S.). K.S.S. is the Beth W. and A. Ross Myers Scholar, in which she is supported through the Patrick C. Walsh Prostate Cancer Research Fund.
Footnotes
Citation Mak TN, Sfanos KS, Brüggemann H. 2013. Draft genome sequences of two strains of Propionibacterium acnes isolated from radical prostatectomy specimens. Genome Announc. 1(6):e01071-13. doi:10.1128/genomeA.01071-13.
REFERENCES
- 1. Perry A, Lambert P. 2011. Propionibacterium acnes: infection beyond the skin. Expert Rev. Anti Infect. Ther. 9:1149–1156 [DOI] [PubMed] [Google Scholar]
- 2. Alexeyev OA, Marklund I, Shannon B, Golovleva I, Olsson J, Andersson C, Eriksson I, Cohen R, Elgh F. 2007. Direct visualization of Propionibacterium acnes in prostate tissue by multicolor fluorescent in situ hybridization assay. J. Clin. Microbiol. 45:3721–3728 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3. Fassi Fehri L, Mak TN, Laube B, Brinkmann V, Ogilvie LA, Mollenkopf H, Lein M, Schmidt T, Meyer TF, Brüggemann H. 2011. Prevalence of Propionibacterium acnes in diseased prostates and its inflammatory and transforming activity on prostate epithelial cells. Int. J. Med. Microbiol. 301:69–78 [DOI] [PubMed] [Google Scholar]
- 4. Mak TN, Yu SH, De Marzo AM, Brüggemann H, Sfanos KS. 2013. Multilocus sequence typing (MLST) analysis of Propionibacterium acnes isolates from radical prostatectomy specimens. Prostate 73:770–777 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5. Lomholt HB, Kilian M. 2010. Population genetic analysis of Propionibacterium acnes identifies a subpopulation and epidemic clones associated with acne. PLoS One 5:e12277 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6. Shinohara DB, Vaghasia AM, Yu SH, Mak TN, Brüggemann H, Nelson WG, De Marzo AM, Yegnasubramanian S, Sfanos KS. 2013. A mouse model of chronic prostatic inflammation using a human prostate cancer-derived isolate of Propionibacterium acnes. Prostate 73:1007–1015 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7. Mak TN, Fischer N, Laube B, Brinkmann V, Metruccio MM, Sfanos KS, Mollenkopf HJ, Meyer TF, Brüggemann H. 2012. Propionibacterium acnes host cell tropism contributes to vimentin-mediated invasion and induction of inflammation. Cell. Microbiol. 14:1720–1733 [DOI] [PubMed] [Google Scholar]