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. Author manuscript; available in PMC: 2013 Dec 24.
Published in final edited form as: Oncogene. 2012 Dec 17;32(42):10.1038/onc.2012.530. doi: 10.1038/onc.2012.530

Figure 1.

Figure 1

Multiple activated tyrosine kinases in human lung adenocarcinomas associated with formation of pY654-β-catenin complexes with HIF1α and p-Smad2. (a) Human lung tumors (T) and contiguous normal tissues (N) were lysed and the lysates immunoprecipitated for pY654-β-catenin and blotted for β-catenin and p-Smad2. Equal amount of protein from both normal and tumor lysates were mixed together as input for mouse IgG control. (b–d) The above lysates were blotted for EMT-related proteins listed in (b), immunoprecipitated for p-c-Met and blotted for total c-Met (c), and sequentially immunoprecipitated for pY654-β-catenin and total β-catenin and blotted for β-catenin, HIF1α, and Src (d). (e) Lysates were sequentially immunoprecipitated for two rounds of HIF1α and one round of pY654-β-catenin and blotted for β-catenin, HIF1α, and Src.