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. 1986 Dec;83(24):9670–9674. doi: 10.1073/pnas.83.24.9670

Identification of the major mannose-binding proteins from chicken egg yolk and chicken serum as immunoglobulins.

K Y Wang, C A Hoppe, P K Datta, A Fogelstrom, Y C Lee
PMCID: PMC387202  PMID: 3467331

Abstract

Chicken egg yolk contains a mannose-binding protein that could be purified with a modification of the procedure of affinity chromatography and gel filtration used for chicken serum mannose-binding protein. The yolk protein was indistinguishable from the serum protein with respect to apparent molecular masses (169 +/- 7 kDa), subunits (74 kDa and 27 kDa, in approximately 1:1 ratio) produced after denaturation in the presence of mercaptoethanol, immunoreactivity with antibody against the chicken serum mannose-binding protein, amino acid composition, pH optimum for binding, calcium independence of binding, sugar-binding specificity, and specific-binding activity. Moreover, the chicken mannose-binding proteins cross-reacted with gamma-chain-specific antibody against chicken IgG. The binding proteins were identified as IgGs by several other criteria: identical electrophoresis pattern when subjected to reducing and nonreducing NaDodSO4/polyacrylamide gel electrophoresis, cochromatography on a Fractogel TSK HW-55(F) column, similar amino acid composition, and isolation of mannose-binding protein from purified serum IgG at a yield comparable to whole serum. These results support the notion that the mannose-binding proteins from the chicken serum and egg yolk are similar, if not identical, and are a subset of chicken IgG.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bidlingmeyer B. A., Cohen S. A., Tarvin T. L. Rapid analysis of amino acids using pre-column derivatization. J Chromatogr. 1984 Dec 7;336(1):93–104. doi: 10.1016/s0378-4347(00)85133-6. [DOI] [PubMed] [Google Scholar]
  2. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  3. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hamazume Y., Mega T., Ikenaka T. Characterization of hen egg white- and yolk-riboflavin binding proteins and amino acid sequence of egg white-riboflavin binding protein. J Biochem. 1984 Jun;95(6):1633–1644. doi: 10.1093/oxfordjournals.jbchem.a134776. [DOI] [PubMed] [Google Scholar]
  5. Hubbard A. L., Wilson G., Ashwell G., Stukenbrok H. An electron microscope autoradiographic study of the carbohydrate recognition systems in rat liver. I. Distribution of 125I-ligands among the liver cell types. J Cell Biol. 1979 Oct;83(1):47–64. doi: 10.1083/jcb.83.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hudgin R. L., Pricer W. E., Jr, Ashwell G., Stockert R. J., Morell A. G. The isolation and properties of a rabbit liver binding protein specific for asialoglycoproteins. J Biol Chem. 1974 Sep 10;249(17):5536–5543. [PubMed] [Google Scholar]
  7. Kawasaki N., Kawasaki T., Yamashina I. Isolation and characterization of a mannan-binding protein from human serum. J Biochem. 1983 Sep;94(3):937–947. doi: 10.1093/oxfordjournals.jbchem.a134437. [DOI] [PubMed] [Google Scholar]
  8. Kawasaki T., Ashwell G. Isolation and characterization of an avian hepatic binding protein specific for N-acetylglucosamine-terminated glycoproteins. J Biol Chem. 1977 Sep 25;252(18):6536–6543. [PubMed] [Google Scholar]
  9. Kawasaki T., Etoh R., Yamashina I. Isolation and characterization of a mannan-binding protein from rabbit liver. Biochem Biophys Res Commun. 1978 Apr 14;81(3):1018–1024. doi: 10.1016/0006-291x(78)91452-3. [DOI] [PubMed] [Google Scholar]
  10. Kawasaki T., Mizuno Y., Masuda T., Yamashina I. Mannan-binding protein in lymphoid tissues of rats. J Biochem. 1980 Dec;88(6):1891–1894. [PubMed] [Google Scholar]
  11. Kozutsumi Y., Kawasaki T., Yamashina I. Isolation and characterization of a mannan-binding protein from rabbit serum. Biochem Biophys Res Commun. 1980 Jul 31;95(2):658–664. doi: 10.1016/0006-291x(80)90836-0. [DOI] [PubMed] [Google Scholar]
  12. Krantz M. J., Holtzman N. A., Stowell C. P., Lee Y. C. Attachment of thioglycosides to proteins: enhancement of liver membrane binding. Biochemistry. 1976 Sep 7;15(18):3963–3968. doi: 10.1021/bi00663a009. [DOI] [PubMed] [Google Scholar]
  13. Kuhlenschmidt T. B., Kuhlenschmidt M. S., Roseman S., Lee Y. C. Binding and endocytosis of glycoproteins by isolated chicken hepatocytes. Biochemistry. 1984 Dec 18;23(26):6437–6444. doi: 10.1021/bi00321a025. [DOI] [PubMed] [Google Scholar]
  14. Kuhlenschmidt T. B., Lee Y. C. Specificity of chicken liver carbohydrate binding protein. Biochemistry. 1984 Jul 31;23(16):3569–3575. doi: 10.1021/bi00311a001. [DOI] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Lee Y. C., Stowell C. P., Krantz M. J. 2-Imino-2-methoxyethyl 1-thioglycosides: new reagents for attaching sugars to proteins. Biochemistry. 1976 Sep 7;15(18):3956–3963. doi: 10.1021/bi00663a008. [DOI] [PubMed] [Google Scholar]
  17. Leslie G. A., Clem L. W. Phylogen of immunoglobulin structure and function. 3. Immunoglobulins of the chicken. J Exp Med. 1969 Dec 1;130(6):1337–1352. doi: 10.1084/jem.130.6.1337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Loeken M. R., Roth T. F. Analysis of maternal IgG subpopulations which are transported into the chicken oocyte. Immunology. 1983 May;49(1):21–28. [PMC free article] [PubMed] [Google Scholar]
  19. Maynard Y., Baenziger J. U. Characterization of a mannose and N-acetylglucosamine-specific lectin present in rat hepatocytes. J Biol Chem. 1982 Apr 10;257(7):3788–3794. [PubMed] [Google Scholar]
  20. Maynard Y., Baenziger J. U. Oligosaccharide specific endocytosis by isolated rat hepatic reticuloendothelial cells. J Biol Chem. 1981 Aug 10;256(15):8063–8068. [PubMed] [Google Scholar]
  21. Miller M. S., Bruch R. C., White H. B., 3rd Carbohydrate compositional effects on tissue distribution of chicken riboflavin-binding protein. Biochim Biophys Acta. 1982 Mar 15;715(1):126–136. doi: 10.1016/0304-4165(82)90058-7. [DOI] [PubMed] [Google Scholar]
  22. Mizuno Y., Kozutsumi Y., Kawasaki T., Yamashina I. Isolation and characterization of a mannan-binding protein from rat liver. J Biol Chem. 1981 May 10;256(9):4247–4252. [PubMed] [Google Scholar]
  23. Mori K., Kawasaki T., Yamashina I. Identification of the mannan-binding protein from rat livers as a hepatocyte protein distinct from the mannan receptor on sinusoidal cells. Arch Biochem Biophys. 1983 Apr 15;222(2):542–552. doi: 10.1016/0003-9861(83)90552-0. [DOI] [PubMed] [Google Scholar]
  24. Oka S., Kawasaki T., Yamashina I. Isolation and characterization of mannan-binding proteins from chicken liver. Arch Biochem Biophys. 1985 Aug 15;241(1):95–105. doi: 10.1016/0003-9861(85)90366-2. [DOI] [PubMed] [Google Scholar]
  25. Roberts D. D., Goldstein I. J. Adenine binding sites of the lectin from lima beans (Phaseolus lunatus). J Biol Chem. 1983 Nov 25;258(22):13820–13824. [PubMed] [Google Scholar]
  26. Roberts D. D., Goldstein I. J. Hydrophobic binding properties of the lectin from lima beans (Phaseolus lunatus). J Biol Chem. 1982 Oct 10;257(19):11274–11277. [PubMed] [Google Scholar]
  27. Rose M. E., Orlans E., Buttress N. Immunoglobulin classes in the hen's egg: their segregation in yolk and white. Eur J Immunol. 1974 Jul;4(7):521–523. doi: 10.1002/eji.1830040715. [DOI] [PubMed] [Google Scholar]
  28. Shepherd V. L., Lee Y. C., Schlesinger P. H., Stahl P. D. L-Fucose-terminated glycoconjugates are recognized by pinocytosis receptors on macrophages. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1019–1022. doi: 10.1073/pnas.78.2.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Stowell C. P., Kuhlenschmidt T. B., Hoppe C. A. A fluorescamine assay for submicrogram quantities of protein in the presence of Triton X-100. Anal Biochem. 1978 Apr;85(2):572–580. doi: 10.1016/0003-2697(78)90256-7. [DOI] [PubMed] [Google Scholar]
  30. Stults N. L., Lin P., Hardy M., Lee Y. C., Uchida Y., Tsukada Y., Sugimori T. Immobilization of proteins on partially hydrolyzed agarose beads. Anal Biochem. 1983 Dec;135(2):392–400. doi: 10.1016/0003-2697(83)90701-7. [DOI] [PubMed] [Google Scholar]
  31. Townsend R., Stahl P. Isolation and characterization of a mannose/N-acetylglucosamine/fucose-binding protein from rat liver. Biochem J. 1981 Jan 15;194(1):209–214. doi: 10.1042/bj1940209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wang K. Y., Kuhlenschmidt T. B., Lee Y. C. Isolation and characterization of the major mannose-binding protein in chicken serum. Biochemistry. 1985 Oct 8;24(21):5932–5938. doi: 10.1021/bi00342a036. [DOI] [PubMed] [Google Scholar]
  33. Wileman T. E., Lennartz M. R., Stahl P. D. Identification of the macrophage mannose receptor as a 175-kDa membrane protein. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2501–2505. doi: 10.1073/pnas.83.8.2501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wong W. S., Osuga D. T., Feeney R. E. Pyridine borane as a reducing agent for proteins. Anal Biochem. 1984 May 15;139(1):58–67. doi: 10.1016/0003-2697(84)90388-9. [DOI] [PubMed] [Google Scholar]
  35. Yamashita K., Kamerling J. P., Kobata A. Structural studies of the sugar chains of hen ovomucoid. Evidence indicating that they are formed mainly by the alternate biosynthetic pathway of asparagine-linked sugar chains. J Biol Chem. 1983 Mar 10;258(5):3099–3106. [PubMed] [Google Scholar]
  36. Yamashita K., Kamerling J. P., Kobata A. Structural study of the carbohydrate moiety of hen ovomucoid. Occurrence of a series of pentaantennary complex-type asparagine-linked sugar chains. J Biol Chem. 1982 Nov 10;257(21):12809–12814. [PubMed] [Google Scholar]
  37. Yamashita K., Tachibana Y., Kobata A. The structures of the galactose-containing sugar chains of ovalbumin. J Biol Chem. 1978 Jun 10;253(11):3862–3869. [PubMed] [Google Scholar]

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