MICROBIOLOGY. For the article “Analysis of an orf virus chemokine-binding protein: Shifting ligand specificities among a family of poxvirus viroceptors,” by Bruce T. Seet, Catherine A. McCaughan, Tracy M. Handel, Andrew Mercer, Craig Brunetti, Grant McFadden, and Stephen B. Fleming, which appeared in issue 25, December 9, 2003, of Proc. Natl. Acad. Sci. USA (100, 15137-15142; first published December 1, 2003; 10.1073/pnas.2336648100), the authors note the following errors in Table 1. In the “Chemokine” column, MIP-1α and MIP-1β have inadvertently been switched. Also, in the “Kd, nM” column, the value for MIP-1β should read 0.331 instead of 0.032. The corrected table appears below.
Table 1. Kinetic binding parameters of ORFV NZ2 CBP to various human chemokines.
Chemokine | kon × 107, M−1·s−1 | koff × 10−3, s−1 | Kd, nM |
---|---|---|---|
CC-chemokines | |||
Eotaxin | 0.56 ± 0.02 | 0.05 ± .003 | 0.008 |
MCP-3 | 0.71 ± 0.14 | 0.29 ± 0.08 | 0.043 |
MCP-1 | 1.02 ± 0.23 | 1.86 ± 0.13 | 0.186 |
MIP-1β | 0.64 ± 0.13 | 2.12 ± 0.36 | 0.331 |
MIP-1α | 2.02 ± 0.39 | 11.99 ± 4.03 | 0.583 |
I-309 | 0.23 ± 0.08 | 20.27 ± 5.69 | 9.25 |
MDC | NB | ||
TARC | NB | ||
C-chemokine | |||
Lymphotactin | 1.33 ± 0.48 | 8.01 ± 3.02 | 0.598 |
Values represent mean ± SD and were obtained from global fitting analysis of four different concentrations, each performed in triplicate. Sensorgrams were generated by observing the association and dissociation phases of chemokines binding immobilized ORFV NZ2 CBP. Chemokines that did not bind are indicated by NB. CX3C-chemokine (fractalkine) and CXC-chemokines [murine stromal cell-derived factor (SDF)-1, human SDF-1 α and β, and IL-8] did not bind and are not shown. MDC, monocyte-derived chemokine; TARC, thymus- and activation-regulated chemokine.