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. Author manuscript; available in PMC: 2014 Nov 4.
Published in final edited form as: Vaccine. 2013 Sep 16;31(46):10.1016/j.vaccine.2013.09.010. doi: 10.1016/j.vaccine.2013.09.010

Figure 4. Induction of serum Abs cross-reactive with V3 peptides of B and non-B subtypes by the gp120JRFL/654 complex.

Figure 4

Figure 4

(A) Sera from mice immunized with the gp120JRFL/654 complex (upper panel) or gp120JRFL (bottom panel) was tested for reactivity against a panel of subtype B V3 peptides. (B) Reactivity of sera from mice immunized with the gp120JRFL/654 complex (upper panel) or gp120JRFL alone (lower panel) against V3 peptides of B and non-B subtypes. For control, sera of animals injected with adjuvant only were tested in parallel and used to calculate the cut-off for positive reactivity (mean + 3 standard deviation, depicted as horizontal lines). Peptides were either covalently linked to Nunc plates (panel A) or coated using the standard protocol onto the 96-microwell plates (panel B) and reacted with sera at a 1:100 dilution. Alkaline phosphatase-conjugated anti-mouse IgG was used as the secondary Ab. Means and standard deviations from one of two independent experiments are shown. OD405, optical density at 405 nm.