Table 1.

Neutralizing and binding activities of serum Abs from mice immunized with gp120 versus gp120/654

Mice immunized with	HIV-1 isolate	Tiera	% Neutralizationb	Ab binding to V3c	Ab binding to gp120d	Ab binding to native Envf
gp120JRFL	SF162	1A	51.9 ± 4.4	+e	+++	Yes
	MN	1A	51.0 ±3.8	+++	+	nt
	HXB-2	1B	≤20	−	++	nt
	SS1196.1	1B	≤20	+	++	nt
	6535	1B	≤20	++	++	nt
	3988.25	1B	≤20	−	++	nt
	JRFL	2	≤20	++	+++	No
	YU2	2	≤20	++	++	nt
	WITO	2	≤20	++	+++	nt
	TRJO	3	≤20	−	++	nt
gp120JRFL/654	SF162	1A	88.0 ±4.6	+++	+++	Yes
	MN	1A	78.0 ±2.8	+++	++	nt
	HXB-2	1B	28.3 ±3.2	+	++	nt
	SS1196.1	1B	70.0 ±2.3	+++	++	nt
	6535	1B	42.0 ±4.0	+++	+++	nt
	3988.25	1B	56.1 ±1.8	+	++	nt
	JRFL	2	≤20	+++	+++	No
	YU2	2	≤20	++	++	nt
	WITO	2	≤20	+++	++	nt
	TRJO	3	28.4 ±2.3	++	++	nt

^aAs previously defined in (36).

^bNeutralization of pseudoviruses with different HIV envelopes by sera (1:50 dilution) collected at two weeks after the last immunization in the standard TZM/bl assay. Neutralization above 50% is shown in bold.

^cELISA reactivity against V3 peptides of the corresponding viruses at 1:100 serum dilution.

^dELISA reactivity against gp120 proteins from the corresponding viruses at 1:500 serum dilution.

^eAverage OD₄₀₅ values are summarized as follows: (−) 0.2–0.5; (+) 0.5–1.0; (++) 1.0–2.5; (+++) 2.5–3.5.

^fBinding to membrane-bound HIV envelopes on transfected 293T cells as measured by flow cytometry; nt: not tested