Figure 5. Burn injury is associated with alterations in transcription of Glucokinase (GK) and Glucose-6-Phosphatase (G-6-P).
Total RNA was isolated from liver tissue of both burned and sham-burned and GK (A) and G-6-P (B) mRNA was assayed by real-time RT-PCR, with 18S ribosomal RNA (18S) used as endogenous control. Mean value of the target was divided by the mean value of the endogenous control to obtain a normalized mean quantity per sample. Results shown represent four different animals per group. Bars represent means; error bars correspond to S.E.M. Asterisks denote statistical significance: p < 0.05 for every comparison between groups.