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. 1996 Jul 23;93(15):7486–7490. doi: 10.1073/pnas.93.15.7486

Use of engineered ribozymes to catalyze chimeric gene assembly.

S Mikheeva 1, K A Jarrell 1
PMCID: PMC38771  PMID: 8755500

Abstract

We report the use of engineered ribozymes to shuffle exon cassettes in vitro. Specifically, we have designed derivatives of a group II intron that insert into selected sites in the human tissue plasminogen activator (t-PA) mRNA. The insertion reaction links t-PA sequences to the group II intron sequences so that trans-splicing reactions catalyzed by the intron can be employed to shuffle the t-PA sequences. We expect these results to be generalizable, so that similar ribozymes can be designed to target any desired 13 nucleotide sequence. In principle, the reactions we describe here should be able to link any RNA molecule to any other RNA molecule at any selected point.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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