Abstract
HL-A antigens comprising 11 different antigenic specificities were isolated after papain solubilization of spleen-cell membrane constituents. During the entire purification procedure, β2-microglobulin appeared together with the HL-A antigens. The highly purified antigens were composed of two polypeptide chains. The large subunit carried the antigenic specificity whereas the small polypeptide chain was very similar, if not identical, to β2-microglobulin. The two HL-A antigen polypeptide chains were held together by noncovalent interactions only, and β2-microglobulin, isolated from urine, could replace the small subunit in forming a complex with the large polypeptide chain. The topographical relationship in the cell membrane between β2-microglobulin and the large HL-A antigen polypeptide chain is unknown. The two polypeptide chains may be fortuitously bound as a result of the solubilization procedure.
Keywords: polypeptide chains, spleen-cell membranes, histocompatibility determinants
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cresswell P., Turner M. J., Strominger J. L. Papain-solubilized HL-A antigens from cultured human lymphocytes contain two peptide fragments. Proc Natl Acad Sci U S A. 1973 May;70(5):1603–1607. doi: 10.1073/pnas.70.5.1603. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies D. A., Boyse E. A., Old L. J., Stockert E. Mouse isoantigens: separation of soluble TL (thymus-leukemia) antigen from soluble H-2 histocompatibility antigen by column chromatography. J Exp Med. 1967 Apr 1;125(4):549–558. doi: 10.1084/jem.125.4.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evrin P. E., Peterson P. A., Wide L., Berggård I. Radioimmunoassay of 2 -microglobulin in human biological fluids. Scand J Clin Lab Invest. 1971 Dec;28(4):439–443. doi: 10.3109/00365517109095721. [DOI] [PubMed] [Google Scholar]
- Gally J. A., Edelman G. M. The genetic control of immunoglobulin synthesis. Annu Rev Genet. 1972;6:1–46. doi: 10.1146/annurev.ge.06.120172.000245. [DOI] [PubMed] [Google Scholar]
- HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
- Hütteroth T. H., Cleve H., Litwin S. D., Poulik M. D. The relationship between 2 -microglobulin and immunoglobulin in cultured human lymphoid cell lines. J Exp Med. 1973 Mar 1;137(3):838–843. doi: 10.1084/jem.137.3.838. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kahan B. D., Reisfeld R. A. Advances in the chemistry of transplantation antigens. Transplant Proc. 1969 Mar;1(1):483–488. [PubMed] [Google Scholar]
- Miyakawa Y., Tanigaki N., Kreiter V. P., Moore G. E., Pressman D. Characterization of soluble substances in plasma carrying HL-A alloantigenic activity and HL-A common antigenic activity. Transplantation. 1973 Mar;15(3):312–319. doi: 10.1097/00007890-197303000-00008. [DOI] [PubMed] [Google Scholar]
- Nathenson S. G. Biochemical properties of histocompatibility antigens. Annu Rev Genet. 1970;4(0):69–90. doi: 10.1146/annurev.ge.04.120170.000441. [DOI] [PubMed] [Google Scholar]
- Neville D. M., Jr Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem. 1971 Oct 25;246(20):6328–6334. [PubMed] [Google Scholar]
- Nilsson K., Evrin P. E., Berggård I., Pontén J. Involvement of lymphoid and nonlymphoid cells in the production of beta2-microglobulin-a homologue of the constant domains of IgG. Nat New Biol. 1973 Jul 11;244(132):44–45. doi: 10.1038/newbio244044a0. [DOI] [PubMed] [Google Scholar]
- Nilsson S. F., Peterson P. A. Evidence for multiple thyroxine-binding sites in human prealbumin. J Biol Chem. 1971 Oct 10;246(19):6098–6105. [PubMed] [Google Scholar]
- Peterson P. A., Cunningham B. A., Berggård I., Edelman G. M. 2 -Microglobulin--a free immunoglobulin domain. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1697–1701. doi: 10.1073/pnas.69.7.1697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peterson P. A., Evrin P. E., Berggård I. Differentiation of glomerular, tubular, and normal proteinuria: determinations of urinary excretion of beta-2-macroglobulin, albumin, and total protein. J Clin Invest. 1969 Jul;48(7):1189–1198. doi: 10.1172/JCI106083. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peterson P. A. Isolation and partial characterization of a human vitamin D-binding plasma protein. J Biol Chem. 1971 Dec 25;246(24):7748–7754. [PubMed] [Google Scholar]
- Reisfeld R. A., Kahan B. D. Transplantation antigens. Adv Immunol. 1970;12:117–200. doi: 10.1016/s0065-2776(08)60169-8. [DOI] [PubMed] [Google Scholar]
- Reisfeld R. A., Pellegrino M. A., Kahan B. D. Salt extraction of soluble HL-A antigens. Science. 1971 Jun 11;172(3988):1134–1136. doi: 10.1126/science.172.3988.1134. [DOI] [PubMed] [Google Scholar]
- Sanderson A. R. HL-A substances from human spleens. Nature. 1968 Oct 12;220(5163):192–195. doi: 10.1038/220192a0. [DOI] [PubMed] [Google Scholar]
- Schwartz B. D., Kato K., Cullen S. E., Nathenson S. G. H-2 histocompatibility alloantigens. Some biochemical properties of the molecules solubilized by NP-40 detergent. Biochemistry. 1973 May 22;12(11):2157–2164. doi: 10.1021/bi00735a023. [DOI] [PubMed] [Google Scholar]
- Smithies O., Poulik M. D. Initiation of protein synthesis at an unusual position in an immunoglobulin gene? Science. 1972 Jan 14;175(4018):187–189. doi: 10.1126/science.175.4018.187. [DOI] [PubMed] [Google Scholar]
- Vahlquist A., Peterson P. A. Combination of specific antibodies with the human vitamin A-transporting protein complex. J Biol Chem. 1973 Jun 10;248(11):4040–4046. [PubMed] [Google Scholar]