Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Jan;71(1):52–56. doi: 10.1073/pnas.71.1.52

Two Separate Genes Controlling Stimulation in Mixed Lymphocyte Reaction in Man

Bo Dupont 1,2,3, Robert A Good 1,2,3, Grete S Hansen 1,2,3, Casper Jersild 1,2,3, Lillian Staub Nielsen 1,2,3, Ben H Park 1,2,3, Arne Svejgaard 1,2,3, Mogens Thomsen 1,2,3, Edmond J Yunis 1,2,3
PMCID: PMC387930  PMID: 4129803

Abstract

The genetic control of strong stimulation in the mixed lymphocyte culture reaction is determined by a separate gene (MLR-S) closely linked to the FOUR-locus of the HL-A chromosomal region. Three additional examples of siblings with recombination between FOUR-locus and MLR-S locus are presented which confirms the independent genetic control of mixed lymphocyte reaction from the control of HL-A antigens. The occurrence of two recombinant children in one family with four other children representing all possible HL-A haplotype-combinations, strongly supports the genetic mapping of the MLR-S determinants outside the HL-A chromosomal region. The experiments presented show that additional genes located within the HL-A region itself contribute with a weak stimulation of allogenic mixtures. These data are discussed in relation to the marginal stimulation of the mixed lymphocyte culture reaction which can be seen between unrelated individuals. It seems that a group of relatively histocompatible individuals can be defined by identity of the MLR-S locus but with differences on the weak MLC-determinants, and that this group for the purpose of clinical transplantation behaves as histocompatible individuals.

Full text

PDF
52

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach F. H., Amos D. B. Hu-1: Major histocompatibility locus in man. Science. 1967 Jun 16;156(3781):1506–1508. doi: 10.1126/science.156.3781.1506. [DOI] [PubMed] [Google Scholar]
  2. Bach F. H. The major histocompatibility complex in transplantation immunology. Transplant Proc. 1973 Mar;5(1):23–29. [PubMed] [Google Scholar]
  3. Bach F. H., Widmer M. B., Bach M. L., Klein J. Serologically defined and lymphocyte-defined components of the major histocompatibility complex in the mouse. J Exp Med. 1972 Dec 1;136(6):1430–1444. doi: 10.1084/jem.136.6.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benacerraf B., McDevitt H. O. Histocompatibility-linked immune response genes. Science. 1972 Jan 21;175(4019):273–279. doi: 10.1126/science.175.4019.273. [DOI] [PubMed] [Google Scholar]
  5. Dupont B., Andersen V., Ernst P., Faber V., Good R. A., Hansen G. S., Henriksen K., Jensen K., Juhl F., Killmann S. A. Immunologic reconstitution in severe combined immunodeficiency with HL-A-incompatible bone marrow graft: donor selection by mixed lymphocyte culture. Transplant Proc. 1973 Mar;5(1):905–908. [PubMed] [Google Scholar]
  6. Dupont B., Andersen V., Hansen G. S., Svejgaard A. Abnormal mixed lymphocyte reactions in the family of a patient with severe combined immunodeficiency. Transplantation. 1972 Nov;14(5):557–563. doi: 10.1097/00007890-197211000-00005. [DOI] [PubMed] [Google Scholar]
  7. Eijsvoogel V. P., de Groot-Kooy L., Huismans L., van Rood J. J., van Leeuwen A., DuToit E. D. Mixed lymphocyte culture and HL-A. Transplant Proc. 1972 Jun;4(2):199–204. [PubMed] [Google Scholar]
  8. Festenstein H., Abbasi K., Sachs J. A., Oliver R. T. Serologically undetectable immune responses in transplantation. Transplant Proc. 1972 Jun;4(2):219–222. [PubMed] [Google Scholar]
  9. Mempel W., Albert E., Burger A. Further evidence for a separate MLC-locus. Tissue Antigens. 1972;2(3):250–254. doi: 10.1111/j.1399-0039.1972.tb00142.x. [DOI] [PubMed] [Google Scholar]
  10. Sasportes M., Lebrun A., Rapaport F. T., Dausset J. Studies of skin allograft survival and mixed lymphocyte culture reaction in Hl-A-genotyped families. Transplant Proc. 1972 Jun;4(2):209–218. [PubMed] [Google Scholar]
  11. Sorensen S. F., Nielsen L. S. The genetic basis for reactivity in human mixed lymphocyte culture. 2. Studies of unrelated subjects identical at the HL-A subloci "LA" and "Four". Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(6):719–725. [PubMed] [Google Scholar]
  12. Yunis E. J., Amos D. B. Three closely linked genetic systems relevant to transplantation. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3031–3035. doi: 10.1073/pnas.68.12.3031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yunis E. J., Plate J. M., Ward F. E., Seigler H. F., Amos D. B. Anomalous MLR responsiveness among siblings. Transplant Proc. 1971 Mar;3(1):118–120. [PubMed] [Google Scholar]
  14. van Rood J. J., Eijsvoogel V. P. HL-A identical phenotypes and genotypes in unrelated individuals. Lancet. 1970 Apr 4;1(7649):698–700. doi: 10.1016/s0140-6736(70)90927-x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES