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. 1974 Jan;71(1):73–77. doi: 10.1073/pnas.71.1.73

Penetration of Mouse Fibroblasts by the 5′-Phosphate of 9-β-D-Arabinofuranosyladenine and Incorporation of the Nucleotide into DNA*

William Plunkett 1, Lillie Lapi 1, Priscilla J Ortiz 1, Seymour S Cohen 1
PMCID: PMC387934  PMID: 4359332

Abstract

9-β-D-Arabinofuranosyladenine 5′-monophosphate (araAMP) is more lethal to mouse fibroblasts (L cells) than the identical exogenous concentration of 9-β-D-arabinofuranosyladenine (araA) (Cancer Res. 32, 1512, 1972). [3H,32P]AraAMP (0.1 mM) was taken into L cells for 4 hr in the presence of a large excess of 32Pi. The radioactivity was subsequently found mainly in the adenine nucleotides in the acid-soluble fraction and in the cell DNA. The cellular concentration of 9-β-D-arabinofuranosyladenine 5′-triphosphate (araATP) exceeded 2 μM. More than 90% of the 3H was associated with araA in the nucleotides. After degradation of the adenine-containing triphosphates with apyrase, the adenine mononucleotides were separated by thin-layer electrophoresis and chromatography. All of the 32P and 97% of the 3H were associated with araAMP.

The small amounts of 3H and 32P in the acid-insoluble material were similar during a 4 hr incubation. The DNA fraction was degraded enzymatically to 5′-mononucleotides. Both 32P and 3H were associated predominantly with 5′-dAMP. Most of the 3H was in araA, detected after dephosphorylation. Enzymatic degradation of the DNA fraction to 3′-mononucleotides and fractionation revealed 3H primarily in the 3′-adenine nucleotide and 32P in each of the deoxynucleoside 3′-monophosphates. After dephosphorylation of the 3′-mononucleotides, 93% of the 3H was found in araA. These results suggest that small amounts of araAMP penetrated the cell as an intact nucleotide, were further phosphorylated to the triphosphate, and subsequently incorporated in internucleotide linkage into DNA.

Keywords: penetrability, nucleotides, araA, araAMP, araATP

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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