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. 1974 Jan;71(1):84–88. doi: 10.1073/pnas.71.1.84

Insulin-Dependent Regulation of Insulin Receptor Concentrations: A Direct Demonstration in Cell Culture

James R Gavin III *, Jesse Roth *, David M Neville Jr †, Pierre De Meyts *, Donald N Buell ‡
PMCID: PMC387937  PMID: 4359334

Abstract

Chronic (5-16 hr) exposure of cultured human lymphocytes to 10-8 M insulin at 37° in vitro produced a decrease in insulin receptor concentrations unaccounted for by simple occupancy of sites; acute exposure (0-2 hr) was without effect. These results reproduced observations in vivo where chronic hyperinsulinemia (e.g., 10-8 M insulin in the circulation of obese insulinresistant hyperglycemic mice) is associated with a substantial reduction in the concentration of insulin receptors per cell, while acute hyperinsulinemia in vivo has no effect on receptor concentration. These data suggest a reciprocal relationship between insulin in the extracellular fluid and the concentration of insulin receptors per cell, which is mediated at the target cell itself by intracellular insulin-sensitive regulatory processes and directly affects target-cell sensitivity to hormone.

Keywords: lymphocytes, extracellular fluid, hormone

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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