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. 1974 Jan;71(1):89–92. doi: 10.1073/pnas.71.1.89

The Requirement for Ribosomal Proteins L7 and L12 in Peptide-Chain Termination

Nathan Brot *, W P Tate *, C T Caskey *, Herbert Weissbach *
PMCID: PMC387938  PMID: 4589896

Abstract

Proteins L7 and L12 from 50S ribosomal subunits of Escherichia coli are required for peptidechain termination. This termination process is inhibited by thiostrepton. Since both thiostrepton-treated ribosomes and those depleted of L7 and L12 have a markedly reduced ability to form release factor·UA[3H]A·ribosome complexes, the binding of release factors to the ribosome appears to be the primary site of inhibition.

Keywords: E. coli, thiostrepton, ribosomal protein

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Selected References

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