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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Jan;71(1):96–98. doi: 10.1073/pnas.71.1.96

Kinetics of Early Changes in Phosphate and Uridine Transport and Cyclic AMP Levels Stimulated by Serum in Density-Inhibited 3T3 Cells

Luis Jimenez De Asua 1, Enrique Rozengurt 1, Renato Dulbecco 1
PMCID: PMC387940  PMID: 4359335

Abstract

A kinetic study of the early changes in uridine- and phosphate-transport rates and 3′:5′-cyclic AMP levels induced by the addition of serum to quiescent 3T3 cells revealed that the increase in phosphate uptake and the decrease in the intracellular concentration of 3′:5′-cyclic AMP immediately follow the addition of serum. In contrast, the increase in uridine transport occurred after a lag of several minutes and was clearly preceded by the other changes. The activation of phosphate transport involved an increase in Vmax, while a more complex pattern was observed for uridine transport. A possible role of phosphate in connection with the other early events was also studied. The addition of phosphate to quiescent cells produced a time-dependent decrease in 3′:5′-cyclic AMP levels and this ion was required for full activation of uridine transport by serum. The results show that the increase in phosphate transport is a primary event in the reinitiation of growth and suggest that the increase in phosphate transport may be connected with subsequent metabolic steps.

Keywords: fibroblasts, membrane, tissue culture, growth regulation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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