Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Jan;71(1):148–152. doi: 10.1073/pnas.71.1.148

Tumor-Host Cell Hybrids in Radiochimeras

Francis Wiener 1, Eva Maria Fenyö 1, George Klein 1
PMCID: PMC387954  PMID: 4521047

Abstract

F1 hybrid mice syngeneic or semiallogeneic with respect to the relevant tumor were lethally irradiated and then reconstituted with hemopoietic cells from strain CBAT6T6 mice. After chimerism had been established, the animals were inoculated with solid or ascites tumors. Tumor-host cell hybrids were selected from enzyme-deficient solid tumors by explanting the tumor cell suspension into hypoxanthine-amethopterin-thymidine containing medium. The selection of hybrid cells from ascites tumors was achieved by exploiting the difference between the ascites tumor cells and hybrid cells in their ability to adhere to the surface of culture vessels. T6T6 chromosomal and H-2 antigenic markers served to distinguish between the hemopoietic cells derived from the donor graft and the cells of the host. All solid tumors tested fused with cells of the irradiated host, whereas ascites tumors fused with repopulating cells of hemopoietic origin.

Keywords: in vivo hybrid, enzyme-deficient solid tumors, ascites tumors, macrophage

Full text

PDF

Images in this article

151Image
on p.151
152Image
on p.152

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allderdice P. W., Miller O. J., Miller D. A., Warburton D., Pearson P. L., Klein G., Harris H. Chromosome analysis of two related heteroploid mouse cell lines by quinacrine fluorescence. J Cell Sci. 1973 Jan;12(1):263–274. doi: 10.1242/jcs.12.1.263. [DOI] [PubMed] [Google Scholar]
  2. BALNER H. Identification of peritoneal macrophages in mouse radiation chimeras. Transplantation. 1963 Apr;1:217–223. doi: 10.1097/00007890-196301020-00009. [DOI] [PubMed] [Google Scholar]
  3. FORD C. E., HAMERTON J. L., BARNES D. W., LOUTIT J. F. Cytological identification of radiation-chimaeras. Nature. 1956 Mar 10;177(4506):452–454. doi: 10.1038/177452a0. [DOI] [PubMed] [Google Scholar]
  4. GESNER B. M., GOWANS J. L. The fate of lethally irradiated mice given isologous and heterologous thoracic duct lymphocytes. Br J Exp Pathol. 1962 Aug;43:431–440. [PMC free article] [PubMed] [Google Scholar]
  5. GOODMAN J. W. ON THE ORIGIN OF PERITONEAL FLUID CELLS. Blood. 1964 Jan;23:18–26. [PubMed] [Google Scholar]
  6. GOODMAN J. W. TRANSPLANTATION OF PERITONEAL FLUID CELLS. Transplantation. 1963 Jul;1:334–346. doi: 10.1097/00007890-196301030-00010. [DOI] [PubMed] [Google Scholar]
  7. Godleski J. J., Brain J. D. The origin of alveolar macrophages in mouse radiation chimeras. J Exp Med. 1972 Sep 1;136(3):630–643. doi: 10.1084/jem.136.3.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gordon S., Cohn Z. Macrophage-melanocyte heterokaryons. I. Preparation and properties. J Exp Med. 1970 May 1;131(5):981–1003. doi: 10.1084/jem.131.5.981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gordon S., Cohn Z. Macrophage-melanocyte heterokaryons. II. The activation of macrophage DNA synthesis. Studies with inhibitors of RNA synthesis. J Exp Med. 1971 Feb 1;133(2):321–338. doi: 10.1084/jem.133.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gordon S., Cohn Z. Macrophage-melanoma cell heterokaryons. 3. The activation of macrophage DNA synthesis. Studies with inhibitors of protein synthesis and with synchronized melanoma cells. J Exp Med. 1971 Oct 1;134(4):935–946. doi: 10.1084/jem.134.4.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gordon S., Cohn Z. Macrophage-melanoma cell heterokaryons. IV. Unmasking the macrophage-specific membrane receptor. J Exp Med. 1971 Oct 1;134(4):947–962. doi: 10.1084/jem.134.4.947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gordon S., Ripps C. S., Cohn Z. The preparation and properties of macrophage-L cell hybrids. J Exp Med. 1971 Nov 1;134(5):1187–1200. doi: 10.1084/jem.134.5.1187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HELLSTROM K. E. Chromosomal studies on diethylstilbestrol-induced testicular tumors in mice. J Natl Cancer Inst. 1961 Mar;26:707–717. [PubMed] [Google Scholar]
  14. Klein G., Friberg S., Jr, Wiener F., Harris H. Hybrid cells derived from fusion of TA3-Ha ascites carcinoma with normal fibroblasts. 1. Malignancy, karyotype, and formation of isoantigenic variants. J Natl Cancer Inst. 1973 May;50(5):1259–1268. doi: 10.1093/jnci/50.5.1259. [DOI] [PubMed] [Google Scholar]
  15. Klein G., Gars U., Harris H. Isoantigen expression in hybrid mouse cells. Exp Cell Res. 1970 Sep;62(1):149–160. doi: 10.1016/0014-4827(79)90516-0. [DOI] [PubMed] [Google Scholar]
  16. NOWELL P. C., COLE L. J., HABERMEYER J. G., ROAN P. L. Growth and continued function of rat marrow cells in x-radiated mice. Cancer Res. 1956 Mar;16(3):258–261. [PubMed] [Google Scholar]
  17. Pinkett M. O., Cowdrey C. R., Nowell P. C. Mixed hematopoietic and pulmonary origin of 'alveolar macrophages' as demonstrated by chromosome markers. Am J Pathol. 1966 May;48(5):859–867. [PMC free article] [PubMed] [Google Scholar]
  18. ROTHFELS K. H., SIMINOVITCH L. An air-drying technique for flattening chromosomes in mammalian oells grown in vitro. Stain Technol. 1958 Mar;33(2):73–77. doi: 10.3109/10520295809111827. [DOI] [PubMed] [Google Scholar]
  19. Reif A. E. An experimental test of two general relationships to describe the adsorption of antibodies by cells and tissues. Immunochemistry. 1966 Jul;3(4):267–278. doi: 10.1016/0019-2791(66)90090-5. [DOI] [PubMed] [Google Scholar]
  20. Shand F. L., Bell E. B. Studies on the distribution of macrophages derived from rat bone marrow cells in xenogeneic radiation chimaeras. Immunology. 1972 Apr;22(4):549–556. [PMC free article] [PubMed] [Google Scholar]
  21. VAN BEKKUM D. W., VOS O. Immunological aspects of homo- and heterologous bone marrow transplantation in irradiated animals. J Cell Physiol Suppl. 1957 Dec;50(Suppl 1):139–156. doi: 10.1002/jcp.1030500410. [DOI] [PubMed] [Google Scholar]
  22. VOLKMAN A., GOWANS J. L. THE PRODUCTION OF MACROPHAGES IN THE RAT. Br J Exp Pathol. 1965 Feb;46:50–61. [PMC free article] [PubMed] [Google Scholar]
  23. Virolainen M. Hematopoietic origin of macrophages as studied by chromosome markers in mice. J Exp Med. 1968 May 1;127(5):943–952. doi: 10.1084/jem.127.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Watkins J. F., Grace D. M. Studies on the surface antigens of interspecific mammalian cell heterokaryons. J Cell Sci. 1967 Jun;2(2):193–204. doi: 10.1242/jcs.2.2.193. [DOI] [PubMed] [Google Scholar]
  25. Wiener F., Fenyö E. M., Klein G., Harris H. Fusion of tumour cells with host cells. Nat New Biol. 1972 Aug 2;238(83):155–159. doi: 10.1038/newbio238155a0. [DOI] [PubMed] [Google Scholar]
  26. van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES