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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1974 Jan;71(1):237–241. doi: 10.1073/pnas.71.1.237

Int-Constitutive Mutants of Bacteriophage Lambda

Kazunori Shimada 1, Allan Campbell 1,*
PMCID: PMC387973  PMID: 4521054

Abstract

The constitutive production of small amounts of trpB enzyme in an Escherichia coli strain carrying λcI857 prophage within the trpC gene has been examined in derivatives of this strain from which portions of the prophage have been deleted. Enzyme production requires a site (pI) within the prophage close to the left prophage end. Selection for mutants of this lysogen that grow on low concentrations of indole yielded two types of mutations within the prophage: (a) v2-type, in which all phage genes controlled by the major leftward operator are derepressed; and (b) int-c type, in which the only phage gene derepressed is int. The int-c mutations lie in the same part of the prophage as pI. All int-c mutants appear deficient in xis gene function, even when derepressed.

Keywords: lysogenization, regulation, prophage excision, tryptophan synthetase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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