Abstract
Two-dimensional tryptic peptide maps of the nonhistone proteins of purified polyoma virus show marked similarities. Protein P1 is a nondisaggregated, possibly covalent, dimer of the major capsid protein P2, whereas P3 and P4 share several new peptides as well as many of the peptides derived from P2. Extensive use of this kind of processing of viral proteins during the biosynthesis of DNA-containing animal viruses has not been reported previously.
Keywords: peptide maps, virus maturation
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- CASPAR D. L., KLUG A. Physical principles in the construction of regular viruses. Cold Spring Harb Symp Quant Biol. 1962;27:1–24. doi: 10.1101/sqb.1962.027.001.005. [DOI] [PubMed] [Google Scholar]
- Cooper P. D., Summers D. F., Maizel J. V. Evidence for ambiguity in the posttranslational cleavage of poliovirus proteins. Virology. 1970 Jul;41(3):408–418. doi: 10.1016/0042-6822(70)90161-3. [DOI] [PubMed] [Google Scholar]
- Eckhart W. Oncogenic viruses. Annu Rev Biochem. 1972;41(10):503–516. doi: 10.1146/annurev.bi.41.070172.002443. [DOI] [PubMed] [Google Scholar]
- Frearson P. M., Crawford L. V. Polyoma virus basic proteins. J Gen Virol. 1972 Feb;14(2):141–155. doi: 10.1099/0022-1317-14-2-141. [DOI] [PubMed] [Google Scholar]
- Friedmann T., David D. Structural roles of polyoma virus proteins. J Virol. 1972 Oct;10(4):776–782. doi: 10.1128/jvi.10.4.776-782.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedmann T. In vitro reassembly of shell-like particles from disrupted polyoma virus. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2574–2578. doi: 10.1073/pnas.68.10.2574. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HIRS C. H. The oxidation of ribonuclease with performic acid. J Biol Chem. 1956 Apr;219(2):611–621. [PubMed] [Google Scholar]
- Jacobson M. F., Asso J., Baltimore D. Further evidence on the formation of poliovirus proteins. J Mol Biol. 1970 May 14;49(3):657–669. doi: 10.1016/0022-2836(70)90289-5. [DOI] [PubMed] [Google Scholar]
- Kiehn E. D., Holland J. J. Synthesis and cleavage of enterovirus polypeptides in mammalian cells. J Virol. 1970 Mar;5(3):358–367. doi: 10.1128/jvi.5.3.358-367.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Moore C. H., Farron F., Bohnert D., Weissmann C. Possible origin of a minor virus specific protein (A1) in Q-beta particles. Nat New Biol. 1971 Sep 15;234(50):204–206. doi: 10.1038/newbio234204a0. [DOI] [PubMed] [Google Scholar]
- Neville D. M., Jr, Glossmann H. Plasma membrane protein subunit composition. A comparative study by discontinuous electrophoresis in sodium dodecyl sulfate. J Biol Chem. 1971 Oct 25;246(20):6335–6338. [PubMed] [Google Scholar]
- Roblin R., Härle E., Dulbecco R. Polyoma virus proteins. 1. Multiple virion components. Virology. 1971 Sep;45(3):555–566. doi: 10.1016/0042-6822(71)90171-1. [DOI] [PubMed] [Google Scholar]
- Sambrook J. Transformation by polyoma virus and simian virus 40. Adv Cancer Res. 1972;16:141–180. [PubMed] [Google Scholar]
- Vogt V. M., Eisenman R. Identification of a large polypeptide precursor of avian oncornavirus proteins. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1734–1738. doi: 10.1073/pnas.70.6.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]


