Fig. 8.

Kinesin and the Dynactin subunit Glued accumulate near nuclei. (A,D,G) (Left) Immunofluorescence images of the LT muscles (green) and their nuclei (red) in a single hemisegment of a stage 16 embryo. Yellow boxes indicate regions shown at higher magnification to the right. (Right) Higher magnification images of the nuclei and Khc, shown as merged images (red, nuclei; gray, Khc) and as separate signals with Khc shown as a heat map to illustrate regions of accumulation near the nucleus in control embryos and toward the muscle pole in Klc^8ex94 embryos. Scale indicating relative intensities is shown bottom right of G. Green boxes indicate regions used for linescan intensity profiles. (B,E,H) Intensity profiles of Khc in control (B), Khc⁸ (E) and Klc^8ex94 (H) embryos. (C,F,I) The average intensity of the Khc immunofluorescence signal both perinuclear and distant from the nucleus in the cytoplasm of control (C), Khc⁸ (F) and Klc^8ex94 (I) embryos. (J) (Left) Immunofluorescence image of the LT muscles (green) and their nuclei (red) in a single hemisegment in a stage 16 embryo expressing GFP-Glued specifically in the mesoderm. Yellow box indicates the region shown at higher magnification to the right. (Right) Higher magnification images of nuclei and GFP-Glued, shown as merged images (red, nuclei; gray, GFP-Glued) and as separate signals with GFP-Glued shown as a heat map to illustrate regions of accumulation. Green box indicates the region used for the linescan intensity profile. (K) Intensity profile of GFP-Glued in a muscle expressing the transgene. (L) The average intensity of GFP-Glued fluorescence signal both perinuclear and distant from the nucleus in the cytoplasm. For C,F,I,L, error bars are the s.d. from 30 muscles in 15 embryos from three independent experiments. ^**P<0.01, compared with control in C unless otherwise indicated by brackets. Scale bars: 10 μm in A,D,G,J left; 5 μm in A,D,G,J right.