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. 1974 Feb;71(2):263–264. doi: 10.1073/pnas.71.2.263

Transformation by Simian Virus 40 of Spleen Cells from a Hyperimmune Rabbit: Demonstration of Production of Specific Antibody to the Immunizing Antigen

A D Strosberg 1,2,3,4, J J Collins 1,2,3,4, P H Black 1,2,3,4, D Malamud 1,2,3,4, S Wilbert 1,2,3,4, K J Bloch 1,2,3,4, E Haber 1,2,3,4
PMCID: PMC387982  PMID: 4150021

Abstract

Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [125I]S3 polysaccharide-protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti-S3 antibodies of the donor rabbit. These observations strongly suggest that a normal committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.

Keywords: immunoadsorbent, isoelectric focusing, pneumococcal capsular polysaccharide

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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