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. 1974 Feb;71(2):464–468. doi: 10.1073/pnas.71.2.464

Interaction of Mycoplasmas with Cell Cultures, as Visualized by Electron Microscopy

Susan Brown 1,2, Marlyn Teplitz 1,2, Jean-Paul Revel 1,2
PMCID: PMC388027  PMID: 4521817

Abstract

Mycoplasmas were examined on the surfaces of tissue culture cells prepared for transmission and scanning electron microscopy. The pleomorphic bodies seen were proved to be mycoplasmas by the use of thin sections, passage of the infection from one cell line to another, and by autoradiography with [3H]thymidine both on the sections and the replicas. The mycoplasmas were not always evenly distributed over the cell's surface; their arrangement seemed to correlate with the activity or morphology of the cell. The use of replicas and scanning electron microscopy in routine examination of cultures for mycoplasma contamination is discussed.

Keywords: scanning electron microscope, cell-surface replicas, autoradiography

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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