Abstract
Native and recombined lipoproteins have been studied by 31P nuclear magnetic resonance spectroscopy. Very low-, low-, and high-density lipoproteins exhibited characteristic spectra. The main resonances were assigned to phosphatidylcholine and sphingomyelin. Relaxation times for these phospholipids were separately measured in low-density lipoproteins and high-density lipoproteins. The effect of paramagnetic ions (Eu+++) on the nuclear magnetic resonance spectrum of high-density lipoproteins is reported.
Keywords: lipid-protein binding, high- and low-density lipoproteins, europium-lipoprotein interactions, sphingomyelin-phosphorus ratio of lipoproteins, chemical shift and relaxation times of lipoprotein constituents
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