Abstract
The transcription of araBAD operon requires araC activator and cyclic AMP. D-Fucose inhibits ara mRNA synthesis. Our results indicate that the positive control by araC activator is exerted at the level of transcription.
Keywords: E. coli, positive control, L-arabinose, D-fucose, catabolite gene activator
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Selected References
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- Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
- Chen B., de Crombrugghe B., Anderson W. B., Gottesman M. E., Pastan I., Perlman R. L. On the mechanism of action of lac repressor. Nat New Biol. 1971 Sep 15;233(37):67–70. doi: 10.1038/newbio233067a0. [DOI] [PubMed] [Google Scholar]
- Gottesman S., Beckwith J. R. Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene. J Mol Biol. 1969 Aug 28;44(1):117–127. doi: 10.1016/0022-2836(69)90408-2. [DOI] [PubMed] [Google Scholar]
- Greenblatt J., Schleif R. Arabinose C protein: regulation of the arabinose operon in vitro. Nat New Biol. 1971 Oct 6;233(40):166–170. doi: 10.1038/newbio233166a0. [DOI] [PubMed] [Google Scholar]
- JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
- Katz L. Selection of araB and araC mutants of Escherichia coli B-r by resistance to ribitol. J Bacteriol. 1970 May;102(2):593–595. doi: 10.1128/jb.102.2.593-595.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morse D. E., Yanofsky C. Polarity and the degradation of mRNA. Nature. 1969 Oct 25;224(5217):329–331. doi: 10.1038/224329a0. [DOI] [PubMed] [Google Scholar]
- Nakanishi S., Adhya S., Gottesman M. E., Pastan I. In vitro repression of the transcription of gas operon by purified gal repressor. Proc Natl Acad Sci U S A. 1973 Feb;70(2):334–338. doi: 10.1073/pnas.70.2.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Power J., Irr J. Regulatory gene control of transcription of the L-arabinose operon in Escherichia coli. J Biol Chem. 1973 Nov 25;248(22):7806–7810. [PubMed] [Google Scholar]
- Rose J. K., Mosteller R. D., Yanofsky C. Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions. J Mol Biol. 1970 Aug;51(3):541–550. doi: 10.1016/0022-2836(70)90007-0. [DOI] [PubMed] [Google Scholar]
- STENT G. S. THE OPERON: ON ITS THIRD ANNIVERSARY. MODULATION OF TRANSFER RNA SPECIES CAN PROVIDE A WORKABLE MODEL OF AN OPERATOR-LESS OPERON. Science. 1964 May 15;144(3620):816–820. doi: 10.1126/science.144.3620.816. [DOI] [PubMed] [Google Scholar]
- Sheppard D. E., Englesberg E. Further evidence for positive control of the L-arabinose system by gene araC. J Mol Biol. 1967 May 14;25(3):443–454. doi: 10.1016/0022-2836(67)90197-0. [DOI] [PubMed] [Google Scholar]
- Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
- Szybalski W. Initiation and patterns of transcription during phage development. Proc Can Cancer Conf. 1969;8:183–215. [PubMed] [Google Scholar]
- Weber K., Kuter D. J. Reversible denaturation of enzymes by sodium dodecyl sulfate. J Biol Chem. 1971 Jul 25;246(14):4504–4509. [PubMed] [Google Scholar]
- Wilcox G., Singer J., Heffernan L. Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r. J Bacteriol. 1971 Oct;108(1):1–4. doi: 10.1128/jb.108.1.1-4.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmerman S. B., Sandeen D. The ribonuclease activity of crystallized pancreatic deoxyribonuclease. Anal Biochem. 1966 Feb;14(2):269–277. doi: 10.1016/0003-2697(66)90137-0. [DOI] [PubMed] [Google Scholar]