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. 1974 Mar;71(3):648–652. doi: 10.1073/pnas.71.3.648

Membrane Molecules Determined by the H-2 Associated Immune Response Region: Isolation and Some Properties

S E Cullen *, C S David , D C Shreffler , S G Nathenson *
PMCID: PMC388069  PMID: 4132532

Abstract

Membrane associated molecules that are probably glycoproteins could be specifically precipitated from NP-40 detergent solubilized extracts of radiolabeled mouse spleen or lymph node cells by antisera produced in congenic strain combinations differing only in the Ir gene region which is linked to the H-2 genes. These Ir region products were designated Lna (lymph node antigen) to conform to previous serological work.

Sodium dodecyl sulfate—polyacrylamide gel electrophoresis of unreduced specific immune precipitates revealed the presence of a possible dimer form, while reduced samples showed only a single peak equivalent to 30,000 daltons. Thus the Lna molecules are clearly distinct from the H-2D and H-2K molecules, which are about 45,000 daltons. Anti-Lna antibodies of different specificity can be present in a single serum; there were at least two separate antigen molecules present in one haplotype tested.

Keywords: histocompatibility, transplantation antigens

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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