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. 1974 Mar;71(3):685–689. doi: 10.1073/pnas.71.3.685

Human Leukemia-Associated Anti-Nuclear Reactivity

George Klein *,†, Melita Steiner *, Francis Wiener *, Eva Klein *,†
PMCID: PMC388077  PMID: 4595570

Abstract

A brilliant, coarsely granular nuclear antigen was detected by anti-complement immunofluorescence in the nuclei of acute myeloid leukemia myeloblasts. Designated as LANA (leukemia-associated nuclear antigen), the reactivity differs from that of the Epstein-Barr-virus-determined nuclear antigen (EBNA) in immunological specificity and morphological appearance, although it is visualized by the same method. Serum from acute myeloid leukemia patients gave positive reactions in 73% of the cases. In acute lymphatic leukemia, chronic myeloid leukemia, chronic lymphatic leukemia, and Burkitt's lymphoma the sera were positive in 35, 14, 19, and 24%, respectively. Two of five polycythemia and two of eleven myeloma sera were also positive. Among 61 healthy controls, 58 were negative, whereas three showed a diffuse nuclear staining with a different pattern. Among 24 carcinoma patients, 18 were negative, whereas six gave a nuclear staining with a different, diffuse pattern. Sera from 20 patients who had recovered from infectious mononucleosis were all negative. In addition to the blasts of acute myeloid leukemia, a similar reactivity was seen with two Epstein-Barr virus DNA and EBNA-negative African lymphoma biopsies and in a short-lived tissue culture line derived from one of them. LANA could be a fetal or tissue-specific antigen, a virally determined antigen, or a specific form of anti-nuclear reactivity.

Keywords: immunology, neoplasms, anti-complement fluorescence, myeloblasts, lymphomas

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Epstein M. A., Achong B. G., Barr Y. M., Zajac B., Henle G., Henle W. Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). J Natl Cancer Inst. 1966 Oct;37(4):547–559. [PubMed] [Google Scholar]
  2. Falk L. A., Wolfe L. G., Hoekstra J., Deinhardt F. Demonstration of Herpesvirus saimiri-associated antigens in peripheral lymphocytes from infected marmosets during in vitro cultivation. J Natl Cancer Inst. 1972 Feb;48(2):523–530. [PubMed] [Google Scholar]
  3. GOLDWASSER R. A., SHEPARD C. C. Staining of complement and modification of fluorescent antibody procedures. J Immunol. 1958 Feb;80(2):122–131. [PubMed] [Google Scholar]
  4. HINUMA Y., HUMMELER K. Studies on the complement-fixing antigens of poliomyelitis. III. Intracellular development of antigen. J Immunol. 1961 Oct;87:367–375. [PubMed] [Google Scholar]
  5. HINUMA Y., OHTA R., MIYAMOTO T., ISHIDA N. Evaluation of the complement method of fluorescent antibody technique with myxoviruses. J Immunol. 1962 Jul;89:19–26. [PubMed] [Google Scholar]
  6. Jondal M., Klein G. Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. J Exp Med. 1973 Dec 1;138(6):1365–1378. doi: 10.1084/jem.138.6.1365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klein G., Clifford P., Klein E., Stjernswärd J. Search for tumor-specific immune reactions in Burkitt lymphoma patients by the membrane immunofluorescence reaction. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1628–1635. doi: 10.1073/pnas.55.6.1628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  9. Nilsson K. High-frequency establishment of human immunoglobulin-producing lymphoblastoid lines from normal and malignant lymphoid tissue and peripheral blood. Int J Cancer. 1971 Nov 15;8(3):432–442. doi: 10.1002/ijc.2910080311. [DOI] [PubMed] [Google Scholar]
  10. Nonoyama M., Huang C. H., Pagano J. S., Klein G., Singh S. DNA of Epstein-Barr virus detected in tissue of Burkitt's lymphoma and nasopharyngeal carcinoma. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3265–3268. doi: 10.1073/pnas.70.11.3265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rabson A. S., O'Conor G. T., Lorenz D. E., Kirschstein R. L., Legallais F. Y., Tralka T. S. Lymphoid cell-culture line derived from lymph node of marmoset infected wtih Herpesvirus saimiri--preliminary report. J Natl Cancer Inst. 1971 May;46(5):1099–1109. [PubMed] [Google Scholar]
  12. Reedman B. M., Klein G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer. 1973 May;11(3):499–520. doi: 10.1002/ijc.2910110302. [DOI] [PubMed] [Google Scholar]
  13. zur Hausen H., Schulte-Holthausen H., Klein G., Henle W., Henle G., Clifford P., Santesson L. EBV DNA in biopsies of Burkitt tumours and anaplastic carcinomas of the nasopharynx. Nature. 1970 Dec 12;228(5276):1056–1058. doi: 10.1038/2281056a0. [DOI] [PubMed] [Google Scholar]

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