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. 1974 Mar;71(3):774–778. doi: 10.1073/pnas.71.3.774

Requirement of Initiation Factor 3 in the Initiation of Polypeptide Synthesis with N-Acetylphenylalanyl-tRNA

Samuel D Bernal 1,2,3, Benjamin M Blumberg 1,2,3, Tokumasa Nakamoto 1,2,3,*
PMCID: PMC388096  PMID: 4522791

Abstract

Initiation factor 3 is required, along with initiation factors 1 and 2, for the incorporation of N-acetylphenylalanine into polypeptides and the formation of N-acetylphenylalanylpuromycin. Initiation factor 3 also strongly stimulates the binding of N-acetylphenylalanyl transfer RNA to isolated 30S ribosomal subunits. Phosphocellulose fractions of initiation factor 3 were found to catalyze N-acetylphenylalanine incorporation differentially with different synthetic messenger RNAs not containing any codons for N-formylmethionine. The results suggest that ribosomes recognize the initiator codon only through the initiator transfer RNA.

Keywords: protein synthesis, initiator codon selection

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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