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. 1974 Mar;71(3):783–787. doi: 10.1073/pnas.71.3.783

Association of DNA-dependent and -independent Ribonucleoside Triphosphatase Activities with dnaB Gene Product of Escherichia coli

Sue Wickner 1, Michel Wright 1, Jerard Hurwitz 1
PMCID: PMC388098  PMID: 4362633

Abstract

Preparations of E. coli dnaB gene product contain ribonucleoside triphosphatase activity that is stimulated 10-fold by DNA. The products of the triphosphatase activity are nucleoside diphosphates and Pi. The dnaB complementing activity in the ϕX174 DNA-dependent system and these triphosphatase activities copurify over the last 20-fold of an extensive (about 40,000-fold) purification procedure. Acrylamide gel electrophoresis of the purified material shows a single band of protein coincident with eluted dnaB complementing and DNA-dependent and -independent nucleoside triphosphatase activities.

Keywords: DNA replication, ϕX174 DNA, ATP

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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