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. 1974 Mar;71(3):979–983. doi: 10.1073/pnas.71.3.979

Identification of a High Affinity Nuclear Acceptor Site for Estrogen Receptor of Calf Uterus

Giovanni Alfredo Puca 1, Vincenzo Sica 1, Ernesto Nola 1
PMCID: PMC388141  PMID: 4362642

Abstract

By means of affinity chromatography, specific nuclear acceptor sites for estradiol receptors are identified in a fraction that can be solubilized from purified nuclei with 2 M NaCl. Interaction between these acceptor sites and crude or partially purified estradiol receptor shows a high association constant (over 109 M). Receptor-acceptor interaction is dependent on physiological concentrations of 17β-estradiol; it is disrupted by high ionic strength. The nuclear acceptor sites appear to be protein in nature and exist in 5- to 10-fold excess over the estrogen binding sites present in the cytosol. Single- or double-stranded DNA does not bind estrogen-receptor complexes. Acceptor sites appear to be associated with basic nuclear proteins as judged by hydroxyapatite chromatography. The nuclear acceptor sites probably represent less than 0.1% of the purified basic proteins from the nucleus.

Keywords: affinity chromatography, basic proteins, DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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