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. 1974 Apr;71(4):1030–1034. doi: 10.1073/pnas.71.4.1030

Genome Construction Between Bacterial Species In Vitro: Replication and Expression of Staphylococcus Plasmid Genes in Escherichia coli

Annie C Y Chang 1, Stanley N Cohen 1
PMCID: PMC388155  PMID: 4598290

Abstract

Genes carried by EcoRI endonuclease-generated fragments of Staphylococcus plasmid DNA have been covalently joined to the E. coli antibiotic-resistance plasmid pSC101, and the resulting hybrid molecules have been introduced into E. coli by transformation. The newly constructed plasmids replicate as biologically functional units in E. coli, and express genetic information carried by both of the parent DNA molecules. In addition, electron microscope heteroduplex analysis of the recombinant plasmids indicate that they contain DNA sequences derived from E. coli and Staphylococcus aureus. Recombinant molecules can transform other E. coli cells for penicillin-resistance markers originally carried by the staphylococcal plasmid, and can be transferred among E. coli strains by conjugally proficient transfer plasmids.

Keywords: transformation, R plasmid, antibiotic resistance, restriction endonuclease, recombination

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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