INFER mice were subcutaneously infected with 625 L3 N. brasiliensis larvae. Lung (A) and MLN (B) was harvested and homogenized at different days p.i for assessment of IL-9 expression by flow cytometry. Numbers represent the frequencies of total GFP+ cells (left column) or GFP+ among CD4+ T cells (right column).
Total numbers of CD4+ T cells (white bars) and ILC2 cells (black bars) expressing IL-9 (GFP+) in lung (C) or MLN (D) of INFER infected mice as determined by flow cytometry.
Data are represented as mean +/− SEM from one out of three independent experiments with 3–4 mice per day p.i. The p values determined by unpaired two-tailed Student’s t test between days are shown.