Abstract
Lymphoid cells from hyperimmune rabbits producing antibodies to a hapten, incubated in the presence of d-lysergic acid diethylamide, continued to synthesize protein at a normal rate. Isoelectric focusing analysis of the low-molecular-weight protein secreted by the cells incubated with lysergic acid diethylamide indicated two components, with pI's of 4.9 and 5.2. Immune cells not exposed to lysergic acid diethylamide secreted only 7S IgG molecules with an average pI of approximately 7.0.
Keywords: LSD, amino acid analogue, isoelectric focusing
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Selected References
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- Ambrose C. T. Symposium on in vitro studies of the immune response. 3. Biochemical agents affecting the inductive phase of the secondary antibody respose initiated in vitro. Bacteriol Rev. 1966 Jun;30(2):408–417. doi: 10.1128/br.30.2.408-417.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HELMREICH E., KERN M., EISEN H. N. The secretion of antibody by isolated lymph node cells. J Biol Chem. 1961 Feb;236:464–473. [PubMed] [Google Scholar]
- Lopatin D. E., Voss E. W., Jr Fluorescein. Hapten and antibody active-site probe. Biochemistry. 1971 Jan 19;10(2):208–213. doi: 10.1021/bi00778a003. [DOI] [PubMed] [Google Scholar]
- McGuigan J. E., Eisen H. N. Differences in spectral properties and tryptophan content among rabbit anti-2,4-dinitrophenyl antibodies of the gamma G-immunologbulin class. Biochemistry. 1968 May;7(5):1919–1928. doi: 10.1021/bi00845a041. [DOI] [PubMed] [Google Scholar]
- Righetti P., Drysdale J. W. Isoelectric focusing in polyacrylamide gels. Biochim Biophys Acta. 1971 Apr 27;236(1):17–28. doi: 10.1016/0005-2795(71)90144-9. [DOI] [PubMed] [Google Scholar]
- Sonoda S., Schlamowitz M. Studies of 125I trace labeling of immunoglobulin G by chloramine-T. Immunochemistry. 1970 Nov;7(11):885–898. doi: 10.1016/0019-2791(70)90051-0. [DOI] [PubMed] [Google Scholar]
- Voss E. W., Jr, Babb J. E., Metzel P., Winkelhake J. L. In vitro effect of d-lysergic acid diethylamide on immunoglobulin synthesis. Biochem Biophys Res Commun. 1973 Feb 5;50(3):950–956. doi: 10.1016/0006-291x(73)91337-5. [DOI] [PubMed] [Google Scholar]
- Voss E. W., Jr, Bauer D. C. Intracellular biosynthesis of rabbit antibody. J Biol Chem. 1967 Oct 10;242(19):4495–4500. [PubMed] [Google Scholar]
- Voss E. W., Jr, Metzel P., Winkelhake J. L. Incorporation of a lysergic acid diethylamide intermediate into antibody protein in vitro. Mol Pharmacol. 1973 May;9(3):421–425. [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]