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. 1974 Apr;71(4):1093–1097. doi: 10.1073/pnas.71.4.1093

Virus-Specific Messenger RNA on Free and Membrane-Bound Polyribosomes from Cells Infected with Rauscher Leukemia Virus

A L J Gielkens 1, M H L Salden 1, H Bloemendal 1,*
PMCID: PMC388169  PMID: 4524617

Abstract

Cells infected by Rauscher leukemia virus synthesize virus-specific RNA which can be detected by hybridization to the single-stranded DNA copy of the viral RNA. Evidence is provided that virus-specific RNA is present in free and membrane-bound polyribosomes of these cells. The relative content of virus-specific RNA, as measured by hybridization, is 6-10 times less on free polyribosomes than on membrane-bound polyribosomes. The messenger RNA associated with both classes of polyribosomes was characterized by density gradient centrifugation. In addition to a major RNA species identified as 36S RNA, at least 2 minor components in the 14S and 21S region have also been found. There is a striking difference in the distribution of these RNA species between free and membrane-bound polyribosomes.

Keywords: hybridization, complementary DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adelman M. R., Blobel G., Sabatini D. D. An improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes. J Cell Biol. 1973 Jan;56(1):191–205. doi: 10.1083/jcb.56.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ando T. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae. Biochim Biophys Acta. 1966 Jan 18;114(1):158–168. doi: 10.1016/0005-2787(66)90263-2. [DOI] [PubMed] [Google Scholar]
  3. Axel R., Schlom J., Spiegelman S. Evidence for translation of viral-specific RNA in cells of a mouse mammary carcinoma. Proc Natl Acad Sci U S A. 1972 Mar;69(3):535–538. doi: 10.1073/pnas.69.3.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berns A. J., Bloemendal H., Kaufman S. J., Verma I. M. Synthesis of DNA complementary to 14S calf lens crystallin messenger RNA by reverse transcriptase. Biochem Biophys Res Commun. 1973 Jun 8;52(3):1013–1019. doi: 10.1016/0006-291x(73)91038-3. [DOI] [PubMed] [Google Scholar]
  5. Cheung K. S., Smith R. E., Stone M. P., Joklik W. K. Comparison of immature (rapid harvest) and mature Rous sarcoma virus particles. Virology. 1972 Dec;50(3):851–864. doi: 10.1016/0042-6822(72)90439-4. [DOI] [PubMed] [Google Scholar]
  6. Coffin J. M., Temin H. M. Hybridization of Rous sarcoma virus deoxyribonucleic acid polymerase product and ribonucleic acids from chicken and rat cells infected with Rous sarcoma virus. J Virol. 1972 May;9(5):766–775. doi: 10.1128/jvi.9.5.766-775.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fan H., Baltimore D. RNA metabolism of murine leukemia virus: detection of virus-specific RNA sequences in infected and uninfected cells and identification of virus-specific messenger RNA. J Mol Biol. 1973 Oct 15;80(1):93–117. doi: 10.1016/0022-2836(73)90235-0. [DOI] [PubMed] [Google Scholar]
  8. Gielkens A. L., Berns T. J., Bloemendal H. An efficient procedure for the isolation of polyribosomes from tissue culture. Eur J Biochem. 1971 Oct 26;22(4):478–484. doi: 10.1111/j.1432-1033.1971.tb01566.x. [DOI] [PubMed] [Google Scholar]
  9. Leong J. A., Garapin A. C., Jackson N., Fanshier L., Levinson W., Bishop J. M. Virus-specific ribonucleic acid in cells producing rous sarcoma virus: detection and characterization. J Virol. 1972 Jun;9(6):891–902. doi: 10.1128/jvi.9.6.891-902.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Milstein C., Brownlee G. G., Harrison T. M., Mathews M. B. A possible precursor of immunoglobulin light chains. Nat New Biol. 1972 Sep 27;239(91):117–120. doi: 10.1038/newbio239117a0. [DOI] [PubMed] [Google Scholar]
  11. Parsons J. T., Coffin J. M., Haroz R. K., Bromley P. A., Weissmann C. Quantitative determination and location of newly synthesized virus-specific ribonucleic acid in chicken cells infected with Rous sarcoma virus. J Virol. 1973 May;11(5):761–774. doi: 10.1128/jvi.11.5.761-774.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Perry R. P., La Torre J., Kelley D. E., Greenberg J. R. On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochim Biophys Acta. 1972 Mar 14;262(2):220–226. doi: 10.1016/0005-2787(72)90236-5. [DOI] [PubMed] [Google Scholar]
  13. Rosbash M. Formation of membrane-bound polyribosomes. J Mol Biol. 1972 Apr 14;65(3):413–422. doi: 10.1016/0022-2836(72)90198-2. [DOI] [PubMed] [Google Scholar]
  14. Stanners C. P. The effect of cycloheximide on polyribosomes from hamster cells. Biochem Biophys Res Commun. 1966 Sep 8;24(5):758–764. doi: 10.1016/0006-291x(66)90390-1. [DOI] [PubMed] [Google Scholar]
  15. Tsuchida N., Robin M. S., Green M. Viral RNA subunits in cells transformed by RNA tumor viruses. Science. 1972 Jun 30;176(4042):1418–1420. doi: 10.1126/science.176.4042.1418. [DOI] [PubMed] [Google Scholar]
  16. Van Ventrooij W. J., Henshaw E. C., Hirsch C. A. Nutritional effects on the polyribosome distribution and rate of protein synthesis in Ehrlich ascites tumor cells in culture. J Biol Chem. 1970 Nov 25;245(22):5947–5953. [PubMed] [Google Scholar]
  17. Vecchio G., Tsuchida N., Shanmugam G., Green M. Virus-specific messenger RNA and nascent polypeptides in polyribosomes of cells replicating murine sarcoma-leukemia viruses. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2064–2068. doi: 10.1073/pnas.70.7.2064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Verma I. M., Meuth N. L., Bromfeld E., Manly K. F., Baltimore D. Covalently linked RNA-DNA molecule as initial product of RNA tumour virus DNA polymerase. Nat New Biol. 1971 Sep 29;233(39):131–134. doi: 10.1038/newbio233131a0. [DOI] [PubMed] [Google Scholar]
  19. Vogt V. M., Eisenman R. Identification of a large polypeptide precursor of avian oncornavirus proteins. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1734–1738. doi: 10.1073/pnas.70.6.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]

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